miRNA display CGI


Results 1 - 6 of 6 are showing below:
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ID Location Perfect MFE.* RefSeq Acc. strand Start Position R_P_ratio# P value
Predicted miRNA align pattern
12317 3' -44 NC_003323.1 + 3096 0.66 0.847991
Target:  5'- cCCAAgaaaauguAUGGAAcCAGGAGUGGCCugaguCUUu -3'
miRNA:   3'- -GGUU--------UGCUUU-GUUUUCACCGGu----GAAc -5'
12317 3' -44 NC_003323.1 + 341 0.66 0.837158
Target:  5'- gUAAGCGAGGCGAccaGGGcUGGuCCACg-- -3'
miRNA:   3'- gGUUUGCUUUGUU---UUC-ACC-GGUGaac -5'
12317 3' -44 NC_003323.1 + 8557 0.66 0.837158
Target:  5'- gUAAGCGAGGCGAccaGGGcUGGuCCACg-- -3'
miRNA:   3'- gGUUUGCUUUGUU---UUC-ACC-GGUGaac -5'
12317 3' -44 NC_003323.1 + 3482 0.71 0.591283
Target:  5'- gCCGggUGggGgguguguaacCAGAcaAGUGGCCACUUu -3'
miRNA:   3'- -GGUuuGCuuU----------GUUU--UCACCGGUGAAc -5'
12317 3' -44 NC_003323.1 + 3423 1.12 0.001021
Target:  5'- uCCAAACGAAACAAAAGUGGCCACUUGu -3'
miRNA:   3'- -GGUUUGCUUUGUUUUCACCGGUGAAC- -5'
12317 3' -44 NC_003323.1 + 4388 0.67 0.825992
Target:  5'- aCAAGCGAuACAAGuauuGCCugUUGg -3'
miRNA:   3'- gGUUUGCUuUGUUUucacCGGugAAC- -5'
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Note:
      When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.

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TDL, Institute of Biomedical Science, Academia Sinica, Taipei, Taiwan.
Copyright © 2007 TDL. All rights reserved.