Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
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Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 12322 | 5' | -57.6 | NC_003324.1 | + | 20592 | 0.76 | 0.166339 |
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Target: 5'- aUCCGgUgCUGUGGCGCCGucgCCAGUGa -3' miRNA: 3'- -AGGCgAgGGUACUGCGGCua-GGUCAC- -5' |
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| 12322 | 5' | -57.6 | NC_003324.1 | + | 36749 | 0.67 | 0.556551 |
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Target: 5'- aUCCGaugcgaggCCCGUGgaGCGCUGG-CCGGUGu -3' miRNA: 3'- -AGGCga------GGGUAC--UGCGGCUaGGUCAC- -5' |
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| 12322 | 5' | -57.6 | NC_003324.1 | + | 22153 | 0.67 | 0.567166 |
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Target: 5'- gUCCGC-CUCGccGACGCCGAUgUAGg- -3' miRNA: 3'- -AGGCGaGGGUa-CUGCGGCUAgGUCac -5' |
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| 12322 | 5' | -57.6 | NC_003324.1 | + | 30049 | 0.66 | 0.610056 |
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Target: 5'- gCCGCcgCCCAUcACGCCG--CCGGUc -3' miRNA: 3'- aGGCGa-GGGUAcUGCGGCuaGGUCAc -5' |
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| 12322 | 5' | -57.6 | NC_003324.1 | + | 23940 | 0.66 | 0.599285 |
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Target: 5'- gUCCGCUgCUCuacaaGACGCCGG-CCGGa- -3' miRNA: 3'- -AGGCGA-GGGua---CUGCGGCUaGGUCac -5' |
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| 12322 | 5' | -57.6 | NC_003324.1 | + | 25930 | 0.66 | 0.588541 |
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Target: 5'- aUCGCUCCaccacGCGCCGcAUCCAGc- -3' miRNA: 3'- aGGCGAGGguac-UGCGGC-UAGGUCac -5' |
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| 12322 | 5' | -57.6 | NC_003324.1 | + | 18105 | 0.69 | 0.39852 |
|
Target: 5'- aUCGCauggCCCGUGGCGCCGG-CCAa-- -3' miRNA: 3'- aGGCGa---GGGUACUGCGGCUaGGUcac -5' |
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| 12322 | 5' | -57.6 | NC_003324.1 | + | 8674 | 0.74 | 0.210969 |
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Target: 5'- aCCGCgCCCGUGACGC-GGUCCucggcAGUGa -3' miRNA: 3'- aGGCGaGGGUACUGCGgCUAGG-----UCAC- -5' |
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| 12322 | 5' | -57.6 | NC_003324.1 | + | 3601 | 1.09 | 0.000611 |
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Target: 5'- uUCCGCUCCCAUGACGCCGAUCCAGUGg -3' miRNA: 3'- -AGGCGAGGGUACUGCGGCUAGGUCAC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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