Results 1 - 20 of 43 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
12329 | 3' | -55.6 | NC_003324.1 | + | 10758 | 1.1 | 0.000854 |
Target: 5'- cUGCGCCAUAUCGCCGGAGAAAGCGCGg -3' miRNA: 3'- -ACGCGGUAUAGCGGCCUCUUUCGCGC- -5' |
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12329 | 3' | -55.6 | NC_003324.1 | + | 32094 | 0.73 | 0.323497 |
Target: 5'- cUGCGCCucggcucGUCGCCGGuGAggccGAGUGCc -3' miRNA: 3'- -ACGCGGua-----UAGCGGCCuCU----UUCGCGc -5' |
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12329 | 3' | -55.6 | NC_003324.1 | + | 51304 | 0.71 | 0.400355 |
Target: 5'- cGCGgaCCAUcgCGCCGGAGuAAAGCa-- -3' miRNA: 3'- aCGC--GGUAuaGCGGCCUC-UUUCGcgc -5' |
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12329 | 3' | -55.6 | NC_003324.1 | + | 38247 | 0.71 | 0.409585 |
Target: 5'- aGCGCCGgaaugccgAUCGCCaucaggucgGGAGu--GCGCGg -3' miRNA: 3'- aCGCGGUa-------UAGCGG---------CCUCuuuCGCGC- -5' |
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12329 | 3' | -55.6 | NC_003324.1 | + | 50281 | 0.71 | 0.409585 |
Target: 5'- aUGCGCCGccgGUCGaCGGcaAGAAGGCGUu -3' miRNA: 3'- -ACGCGGUa--UAGCgGCC--UCUUUCGCGc -5' |
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12329 | 3' | -55.6 | NC_003324.1 | + | 43795 | 0.71 | 0.428438 |
Target: 5'- gGUGCCGggaaCGCCGGc-AAGGCGCGu -3' miRNA: 3'- aCGCGGUaua-GCGGCCucUUUCGCGC- -5' |
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12329 | 3' | -55.6 | NC_003324.1 | + | 33460 | 0.7 | 0.447793 |
Target: 5'- -cCGCCA-GUgGCCGGAGuu-GCGCGc -3' miRNA: 3'- acGCGGUaUAgCGGCCUCuuuCGCGC- -5' |
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12329 | 3' | -55.6 | NC_003324.1 | + | 38368 | 0.7 | 0.457651 |
Target: 5'- gGuCGCCGUcgUGCgGGAGGAAGUGg- -3' miRNA: 3'- aC-GCGGUAuaGCGgCCUCUUUCGCgc -5' |
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12329 | 3' | -55.6 | NC_003324.1 | + | 16155 | 0.7 | 0.467624 |
Target: 5'- cGCGCCAggcAUCGCUGGcaccGAGGCGgGc -3' miRNA: 3'- aCGCGGUa--UAGCGGCCuc--UUUCGCgC- -5' |
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12329 | 3' | -55.6 | NC_003324.1 | + | 41130 | 0.7 | 0.476694 |
Target: 5'- cGCGCUGcaaggcUAUCGCCGGcucacacucgaacAGcGAGGCGCGu -3' miRNA: 3'- aCGCGGU------AUAGCGGCC-------------UC-UUUCGCGC- -5' |
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12329 | 3' | -55.6 | NC_003324.1 | + | 38164 | 0.7 | 0.477707 |
Target: 5'- gGCGCCGacgcgcuUCGCC--AGAAGGCGCa -3' miRNA: 3'- aCGCGGUau-----AGCGGccUCUUUCGCGc -5' |
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12329 | 3' | -55.6 | NC_003324.1 | + | 53668 | 0.7 | 0.477707 |
Target: 5'- aGUGUCAUGUCGCaaaGGGGGAccGGCaucagGCGg -3' miRNA: 3'- aCGCGGUAUAGCGg--CCUCUU--UCG-----CGC- -5' |
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12329 | 3' | -55.6 | NC_003324.1 | + | 27771 | 0.69 | 0.487897 |
Target: 5'- gGCGCac-AUCGCCaaGGAGAAgcucGGCGCc -3' miRNA: 3'- aCGCGguaUAGCGG--CCUCUU----UCGCGc -5' |
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12329 | 3' | -55.6 | NC_003324.1 | + | 31239 | 0.69 | 0.498188 |
Target: 5'- aGCGCCAgcgcCGCCGGccgcacuGCGCGu -3' miRNA: 3'- aCGCGGUaua-GCGGCCucuuu--CGCGC- -5' |
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12329 | 3' | -55.6 | NC_003324.1 | + | 7166 | 0.69 | 0.540253 |
Target: 5'- cGCGCCAacaguUGgugCGCUGGcAGAAcucacGCGCGg -3' miRNA: 3'- aCGCGGU-----AUa--GCGGCC-UCUUu----CGCGC- -5' |
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12329 | 3' | -55.6 | NC_003324.1 | + | 23169 | 0.69 | 0.540253 |
Target: 5'- gGCGCCcacgGUAUgGUCGGc--GAGCGCGg -3' miRNA: 3'- aCGCGG----UAUAgCGGCCucuUUCGCGC- -5' |
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12329 | 3' | -55.6 | NC_003324.1 | + | 4271 | 0.67 | 0.604222 |
Target: 5'- aUGCGCCAcggcguuucCGCCGGuGGcaagaugucccgcAAGCGCa -3' miRNA: 3'- -ACGCGGUaua------GCGGCCuCU-------------UUCGCGc -5' |
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12329 | 3' | -55.6 | NC_003324.1 | + | 27709 | 0.67 | 0.604222 |
Target: 5'- gUGCGCCucggccuUAUCGCgGGcaccGAAccacuucGGCGCGa -3' miRNA: 3'- -ACGCGGu------AUAGCGgCCu---CUU-------UCGCGC- -5' |
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12329 | 3' | -55.6 | NC_003324.1 | + | 2827 | 0.67 | 0.605319 |
Target: 5'- cUGCGgUAUuuUCGCCGGgcgagGGGGAGCGUc -3' miRNA: 3'- -ACGCgGUAu-AGCGGCC-----UCUUUCGCGc -5' |
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12329 | 3' | -55.6 | NC_003324.1 | + | 23044 | 0.67 | 0.616293 |
Target: 5'- aGCGCCuguUCGCucgcaucgccacCGGAGAcuGGGaCGCGg -3' miRNA: 3'- aCGCGGuauAGCG------------GCCUCU--UUC-GCGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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