Results 1 - 7 of 7 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 1233 | 5' | -47.2 | NC_001271.1 | + | 39276 | 0.66 | 0.946024 |
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Target: 5'- -aCACCAUAGGuGAGACcaUAGGa--- -3' miRNA: 3'- aaGUGGUGUCC-CUCUGaaAUUCaauu -5' |
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| 1233 | 5' | -47.2 | NC_001271.1 | + | 11334 | 0.67 | 0.922221 |
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Target: 5'- cUCACUAaAGGGAGACUUaacGGUUu- -3' miRNA: 3'- aAGUGGUgUCCCUCUGAAau-UCAAuu -5' |
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| 1233 | 5' | -47.2 | NC_001271.1 | + | 39405 | 0.67 | 0.922221 |
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Target: 5'- --gGCUAUGGGGGGuACUUUGGGUUc- -3' miRNA: 3'- aagUGGUGUCCCUC-UGAAAUUCAAuu -5' |
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| 1233 | 5' | -47.2 | NC_001271.1 | + | 76 | 0.67 | 0.922221 |
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Target: 5'- --gGCUAUGGGGGGuACUUUGGGUUc- -3' miRNA: 3'- aagUGGUGUCCCUC-UGAAAUUCAAuu -5' |
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| 1233 | 5' | -47.2 | NC_001271.1 | + | 5982 | 0.7 | 0.764695 |
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Target: 5'- cUCACUAC-GGGAGACUacUUAAGg--- -3' miRNA: 3'- aAGUGGUGuCCCUCUGA--AAUUCaauu -5' |
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| 1233 | 5' | -47.2 | NC_001271.1 | + | 38906 | 0.84 | 0.161521 |
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Target: 5'- aUCACCAaucaUAGGGAGACUUUAAGUg-- -3' miRNA: 3'- aAGUGGU----GUCCCUCUGAAAUUCAauu -5' |
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| 1233 | 5' | -47.2 | NC_001271.1 | + | 26238 | 1.05 | 0.006419 |
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Target: 5'- aUUCACCACAGGGAGACUUUAAGUUAAa -3' miRNA: 3'- -AAGUGGUGUCCCUCUGAAAUUCAAUU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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