Results 1 - 12 of 12 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
12330 | 3' | -62.1 | NC_003324.1 | + | 11451 | 0.66 | 0.397396 |
Target: 5'- uGGUCUCCacuggaacGcGCAGGGGCacauuGCGGucaaGAGCg -3' miRNA: 3'- gUCAGAGG--------C-CGUCCUCGg----CGCC----CUCG- -5' |
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12330 | 3' | -62.1 | NC_003324.1 | + | 2367 | 0.66 | 0.387889 |
Target: 5'- gCAGcCcgCCcGCAGGGgcgggucgcggucGCUGCGGGAGUg -3' miRNA: 3'- -GUCaGa-GGcCGUCCU-------------CGGCGCCCUCG- -5' |
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12330 | 3' | -62.1 | NC_003324.1 | + | 51153 | 0.66 | 0.366845 |
Target: 5'- -cGUCUUCaGGCAGGugaccuGCCgcgucaaccugacgaGCGGGGGUu -3' miRNA: 3'- guCAGAGG-CCGUCCu-----CGG---------------CGCCCUCG- -5' |
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12330 | 3' | -62.1 | NC_003324.1 | + | 31321 | 0.67 | 0.347395 |
Target: 5'- gCAGUgCggCCGGCGgcgcuGGcGCUgGCGGGGGCa -3' miRNA: 3'- -GUCA-Ga-GGCCGU-----CCuCGG-CGCCCUCG- -5' |
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12330 | 3' | -62.1 | NC_003324.1 | + | 34165 | 0.67 | 0.331758 |
Target: 5'- uCGGUCUCauggacagGGC-GGAGCCGCuGGcauuGGGCg -3' miRNA: 3'- -GUCAGAGg-------CCGuCCUCGGCG-CC----CUCG- -5' |
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12330 | 3' | -62.1 | NC_003324.1 | + | 16122 | 0.67 | 0.316644 |
Target: 5'- uGGUC-CUGcGCAGGGcGCUucgguaGCGGGGGCc -3' miRNA: 3'- gUCAGaGGC-CGUCCU-CGG------CGCCCUCG- -5' |
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12330 | 3' | -62.1 | NC_003324.1 | + | 24525 | 0.67 | 0.316644 |
Target: 5'- aCAuUUUCCGGCAGGguccggauGGCCuuGGaGAGCa -3' miRNA: 3'- -GUcAGAGGCCGUCC--------UCGGcgCC-CUCG- -5' |
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12330 | 3' | -62.1 | NC_003324.1 | + | 6103 | 0.68 | 0.274445 |
Target: 5'- -uGUCgUCGGCAGGAGCgGacuGGGcGGCa -3' miRNA: 3'- guCAGaGGCCGUCCUCGgCg--CCC-UCG- -5' |
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12330 | 3' | -62.1 | NC_003324.1 | + | 44884 | 0.7 | 0.225339 |
Target: 5'- aAGUCagCGGUAcgccGGGG-CGCGGGGGCu -3' miRNA: 3'- gUCAGagGCCGU----CCUCgGCGCCCUCG- -5' |
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12330 | 3' | -62.1 | NC_003324.1 | + | 45958 | 0.72 | 0.149328 |
Target: 5'- aAGcCUCCGGgAGGAGCUGauuaCGGGGGa -3' miRNA: 3'- gUCaGAGGCCgUCCUCGGC----GCCCUCg -5' |
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12330 | 3' | -62.1 | NC_003324.1 | + | 16263 | 0.72 | 0.14166 |
Target: 5'- --cUUUCUGGCGGGAGCUGCGGu-GCc -3' miRNA: 3'- gucAGAGGCCGUCCUCGGCGCCcuCG- -5' |
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12330 | 3' | -62.1 | NC_003324.1 | + | 11666 | 1.11 | 0.000171 |
Target: 5'- cCAGUCUCCGGCAGGAGCCGCGGGAGCa -3' miRNA: 3'- -GUCAGAGGCCGUCCUCGGCGCCCUCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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