Results 21 - 36 of 36 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
12334 | 3' | -53.1 | NC_003324.1 | + | 44582 | 0.67 | 0.742827 |
Target: 5'- gCAGCGACacgGCGGCGAGGaacugcccguugcaGAUGuGUg -3' miRNA: 3'- -GUCGUUGa--UGCCGCUCUg-------------CUACuCGu -5' |
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12334 | 3' | -53.1 | NC_003324.1 | + | 33432 | 0.68 | 0.72781 |
Target: 5'- cCGGCAACcACGcacCGGGACGAUGAcCAu -3' miRNA: 3'- -GUCGUUGaUGCc--GCUCUGCUACUcGU- -5' |
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12334 | 3' | -53.1 | NC_003324.1 | + | 21084 | 0.68 | 0.69502 |
Target: 5'- uCGGCGcggccaucAUUGCGGCGAuccGACG-UGAGCu -3' miRNA: 3'- -GUCGU--------UGAUGCCGCU---CUGCuACUCGu -5' |
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12334 | 3' | -53.1 | NC_003324.1 | + | 21287 | 0.68 | 0.69502 |
Target: 5'- uCGGCAGCUcgaaagccagGCGGuUGAGcACGAUcgcGAGCAg -3' miRNA: 3'- -GUCGUUGA----------UGCC-GCUC-UGCUA---CUCGU- -5' |
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12334 | 3' | -53.1 | NC_003324.1 | + | 49517 | 0.69 | 0.669478 |
Target: 5'- uGGC-ACUcucggccguaucgaGCGGCGAGGCGAcGuGCAu -3' miRNA: 3'- gUCGuUGA--------------UGCCGCUCUGCUaCuCGU- -5' |
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12334 | 3' | -53.1 | NC_003324.1 | + | 50862 | 0.69 | 0.661659 |
Target: 5'- cCAGCGACagcAUGGCGAGGUGGUGAcCAu -3' miRNA: 3'- -GUCGUUGa--UGCCGCUCUGCUACUcGU- -5' |
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12334 | 3' | -53.1 | NC_003324.1 | + | 34618 | 0.7 | 0.594454 |
Target: 5'- -cGCAGCUcCGG-GAGGCGcUGGGCGa -3' miRNA: 3'- guCGUUGAuGCCgCUCUGCuACUCGU- -5' |
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12334 | 3' | -53.1 | NC_003324.1 | + | 36248 | 0.7 | 0.594454 |
Target: 5'- gCAGCAGgUcgGCGGCGuGACgGAUGAGa- -3' miRNA: 3'- -GUCGUUgA--UGCCGCuCUG-CUACUCgu -5' |
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12334 | 3' | -53.1 | NC_003324.1 | + | 35034 | 0.7 | 0.567786 |
Target: 5'- gGGCGAUgccgaugaucaggGCGGCGcAGAUGAaGAGCAg -3' miRNA: 3'- gUCGUUGa------------UGCCGC-UCUGCUaCUCGU- -5' |
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12334 | 3' | -53.1 | NC_003324.1 | + | 16520 | 0.71 | 0.554561 |
Target: 5'- gCAGCGgaucggccugugcguGCU-CGGCGAGAgGGUGAGgGg -3' miRNA: 3'- -GUCGU---------------UGAuGCCGCUCUgCUACUCgU- -5' |
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12334 | 3' | -53.1 | NC_003324.1 | + | 45352 | 0.71 | 0.528403 |
Target: 5'- gGGCGACUugaagcagGCGGCGAGcaGCGA-GGGCc -3' miRNA: 3'- gUCGUUGA--------UGCCGCUC--UGCUaCUCGu -5' |
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12334 | 3' | -53.1 | NC_003324.1 | + | 40126 | 0.72 | 0.475519 |
Target: 5'- -cGCAACUACGGUG-GACGGccgacGAGCu -3' miRNA: 3'- guCGUUGAUGCCGCuCUGCUa----CUCGu -5' |
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12334 | 3' | -53.1 | NC_003324.1 | + | 14592 | 0.72 | 0.475519 |
Target: 5'- aAGCGGCcgGCGGCGcAGGCGGU-AGCGu -3' miRNA: 3'- gUCGUUGa-UGCCGC-UCUGCUAcUCGU- -5' |
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12334 | 3' | -53.1 | NC_003324.1 | + | 45826 | 0.72 | 0.496377 |
Target: 5'- gAGgGGCUGCGGCGAGAacaGUGAGg- -3' miRNA: 3'- gUCgUUGAUGCCGCUCUgc-UACUCgu -5' |
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12334 | 3' | -53.1 | NC_003324.1 | + | 45686 | 0.8 | 0.159647 |
Target: 5'- gCAGgGGCU-CGGCGAGGCG-UGAGCAg -3' miRNA: 3'- -GUCgUUGAuGCCGCUCUGCuACUCGU- -5' |
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12334 | 3' | -53.1 | NC_003324.1 | + | 16649 | 1.09 | 0.00174 |
Target: 5'- uCAGCAACUACGGCGAGACGAUGAGCAg -3' miRNA: 3'- -GUCGUUGAUGCCGCUCUGCUACUCGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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