Results 1 - 10 of 10 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
12339 | 3' | -55.1 | NC_003324.1 | + | 55050 | 0.68 | 0.613666 |
Target: 5'- gAGCGAagcagugcGACCUgugcaUUGACCAGGUGcuGGAu -3' miRNA: 3'- -UUGCU--------CUGGA-----AGCUGGUCCGCuuCCU- -5' |
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12339 | 3' | -55.1 | NC_003324.1 | + | 45205 | 0.7 | 0.495148 |
Target: 5'- aGGCGAGAUCaUUGACCGuGGUGcGGGGAa -3' miRNA: 3'- -UUGCUCUGGaAGCUGGU-CCGC-UUCCU- -5' |
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12339 | 3' | -55.1 | NC_003324.1 | + | 34931 | 0.77 | 0.194559 |
Target: 5'- gAGCGGGACCgcaucgcagagcagUUCGACgacggcgagcugCAGGCGAAGGAg -3' miRNA: 3'- -UUGCUCUGG--------------AAGCUG------------GUCCGCUUCCU- -5' |
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12339 | 3' | -55.1 | NC_003324.1 | + | 30870 | 0.68 | 0.614769 |
Target: 5'- gGGCGAGACgUUCGACagcaagauggauacGGCGcAGGAc -3' miRNA: 3'- -UUGCUCUGgAAGCUGgu------------CCGCuUCCU- -5' |
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12339 | 3' | -55.1 | NC_003324.1 | + | 25007 | 0.68 | 0.569754 |
Target: 5'- -uCGAGACaCUgCGACCGGGCGgcGu- -3' miRNA: 3'- uuGCUCUG-GAaGCUGGUCCGCuuCcu -5' |
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12339 | 3' | -55.1 | NC_003324.1 | + | 20653 | 0.78 | 0.153475 |
Target: 5'- gGGCgGAGugCUUCGACCAGGCGGcgaugcuuGGAa -3' miRNA: 3'- -UUG-CUCugGAAGCUGGUCCGCUu-------CCU- -5' |
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12339 | 3' | -55.1 | NC_003324.1 | + | 18619 | 1.07 | 0.001492 |
Target: 5'- cAACGAGACCUUCGACCAGGCGAAGGAa -3' miRNA: 3'- -UUGCUCUGGAAGCUGGUCCGCUUCCU- -5' |
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12339 | 3' | -55.1 | NC_003324.1 | + | 15024 | 0.66 | 0.723129 |
Target: 5'- aGGCGAcGGCaggUCGAUCAuGGCGcAGGAc -3' miRNA: 3'- -UUGCU-CUGga-AGCUGGU-CCGCuUCCU- -5' |
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12339 | 3' | -55.1 | NC_003324.1 | + | 2860 | 0.68 | 0.569754 |
Target: 5'- aGGCGguAGACCUgccaGAUCGGGCGAucaaagaucGGGAg -3' miRNA: 3'- -UUGC--UCUGGAag--CUGGUCCGCU---------UCCU- -5' |
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12339 | 3' | -55.1 | NC_003324.1 | + | 2828 | 0.71 | 0.425296 |
Target: 5'- uGCGGuAUUUUCG-CCGGGCGAGGGGg -3' miRNA: 3'- uUGCUcUGGAAGCuGGUCCGCUUCCU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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