Results 21 - 40 of 44 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
12343 | 5' | -54.8 | NC_003324.1 | + | 46458 | 0.68 | 0.633813 |
Target: 5'- aGGguAGCuUGGCUGuauCCUGCuuaCCGCu -3' miRNA: 3'- -CCguUUG-ACUGACu--GGACGucgGGCG- -5' |
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12343 | 5' | -54.8 | NC_003324.1 | + | 29905 | 0.68 | 0.609504 |
Target: 5'- cGGCGcGCUG-CUGGCCaugcuggacggcGCGGCCCa- -3' miRNA: 3'- -CCGUuUGACuGACUGGa-----------CGUCGGGcg -5' |
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12343 | 5' | -54.8 | NC_003324.1 | + | 10070 | 0.68 | 0.611711 |
Target: 5'- uGGCGuuGCUGGCuUGGuCCaUGCuuGGCUCGCg -3' miRNA: 3'- -CCGUu-UGACUG-ACU-GG-ACG--UCGGGCG- -5' |
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12343 | 5' | -54.8 | NC_003324.1 | + | 46576 | 0.68 | 0.611711 |
Target: 5'- aGGCucucgaacAACUGACUGACCggccugGCAGugaucaaacuuCCgGCa -3' miRNA: 3'- -CCGu-------UUGACUGACUGGa-----CGUC-----------GGgCG- -5' |
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12343 | 5' | -54.8 | NC_003324.1 | + | 22596 | 0.68 | 0.58968 |
Target: 5'- cGGCGGccuuUUGGCUG-CCUGCcgcguugccgAGCcCCGCg -3' miRNA: 3'- -CCGUUu---GACUGACuGGACG----------UCG-GGCG- -5' |
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12343 | 5' | -54.8 | NC_003324.1 | + | 18444 | 0.69 | 0.583096 |
Target: 5'- uGGUGAACaUGACgcagacgaucaugcuUGGCCUGCcaauccucggcGCCCGCu -3' miRNA: 3'- -CCGUUUG-ACUG---------------ACUGGACGu----------CGGGCG- -5' |
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12343 | 5' | -54.8 | NC_003324.1 | + | 28727 | 0.69 | 0.567793 |
Target: 5'- uGGCAccAC-GACgcgcGACaagGCGGCCCGCa -3' miRNA: 3'- -CCGUu-UGaCUGa---CUGga-CGUCGGGCG- -5' |
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12343 | 5' | -54.8 | NC_003324.1 | + | 38966 | 0.69 | 0.535376 |
Target: 5'- aGGCugguAAgaGGCUGaaccGCCUGCAguaucGCCUGCa -3' miRNA: 3'- -CCGu---UUgaCUGAC----UGGACGU-----CGGGCG- -5' |
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12343 | 5' | -54.8 | NC_003324.1 | + | 13001 | 0.69 | 0.534306 |
Target: 5'- cGC-GGCUGGC-GAUCUGCAgggcggcaucaacGCCCGCc -3' miRNA: 3'- cCGuUUGACUGaCUGGACGU-------------CGGGCG- -5' |
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12343 | 5' | -54.8 | NC_003324.1 | + | 29244 | 0.69 | 0.566704 |
Target: 5'- cGGC-GGCUGuucgGGCCUGCaucgccgGGCUCGCg -3' miRNA: 3'- -CCGuUUGACuga-CUGGACG-------UCGGGCG- -5' |
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12343 | 5' | -54.8 | NC_003324.1 | + | 28718 | 0.69 | 0.556925 |
Target: 5'- gGGCAGGCgccGAaacCUGcUCUGCAGuuCCCGCg -3' miRNA: 3'- -CCGUUUGa--CU---GACuGGACGUC--GGGCG- -5' |
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12343 | 5' | -54.8 | NC_003324.1 | + | 20581 | 0.69 | 0.556925 |
Target: 5'- uGGCGAGCggGAUccgGugCUGUGGCgCCGUc -3' miRNA: 3'- -CCGUUUGa-CUGa--CugGACGUCG-GGCG- -5' |
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12343 | 5' | -54.8 | NC_003324.1 | + | 16029 | 0.69 | 0.545039 |
Target: 5'- cGGCGGACUGGCUGgugaugagGCCaaguucgacgacuUGCcGgCCGCg -3' miRNA: 3'- -CCGUUUGACUGAC--------UGG-------------ACGuCgGGCG- -5' |
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12343 | 5' | -54.8 | NC_003324.1 | + | 52323 | 0.7 | 0.524711 |
Target: 5'- cGGCAGGacgaACUGGCCgagUGaUAGCCCGCc -3' miRNA: 3'- -CCGUUUgac-UGACUGG---AC-GUCGGGCG- -5' |
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12343 | 5' | -54.8 | NC_003324.1 | + | 31232 | 0.7 | 0.524711 |
Target: 5'- gGGCAGcuccGCgaGGCaGGCCUGCAGCgCCagGCg -3' miRNA: 3'- -CCGUU----UGa-CUGaCUGGACGUCG-GG--CG- -5' |
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12343 | 5' | -54.8 | NC_003324.1 | + | 32886 | 0.7 | 0.468686 |
Target: 5'- aGGCucACUGACaccgucaaucuucGACCUGCGGuUCUGCa -3' miRNA: 3'- -CCGuuUGACUGa------------CUGGACGUC-GGGCG- -5' |
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12343 | 5' | -54.8 | NC_003324.1 | + | 35554 | 0.7 | 0.482931 |
Target: 5'- uGCGAGCgGAuCUGcaagGCCUGC-GCCUGCg -3' miRNA: 3'- cCGUUUGaCU-GAC----UGGACGuCGGGCG- -5' |
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12343 | 5' | -54.8 | NC_003324.1 | + | 54833 | 0.7 | 0.524711 |
Target: 5'- cGGCAGGgaGAUUG-CC-GCAGCCCu- -3' miRNA: 3'- -CCGUUUgaCUGACuGGaCGUCGGGcg -5' |
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12343 | 5' | -54.8 | NC_003324.1 | + | 7420 | 0.71 | 0.462647 |
Target: 5'- gGGCAcggaGAUUGGCaaggGugCUGCuGCCCGa -3' miRNA: 3'- -CCGU----UUGACUGa---CugGACGuCGGGCg -5' |
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12343 | 5' | -54.8 | NC_003324.1 | + | 8514 | 0.74 | 0.295937 |
Target: 5'- cGCAAAUccGcCUGcACCUGCAGCCgGCg -3' miRNA: 3'- cCGUUUGa-CuGAC-UGGACGUCGGgCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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