Results 1 - 13 of 13 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
12344 | 5' | -55.2 | NC_003324.1 | + | 54798 | 0.68 | 0.620826 |
Target: 5'- aGAUGCGcCGAUCGAGCGU--UCg-- -3' miRNA: 3'- gCUACGCcGCUAGCUCGCGcaAGagu -5' |
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12344 | 5' | -55.2 | NC_003324.1 | + | 52992 | 0.72 | 0.414493 |
Target: 5'- aCGAUGCGGCGGaacucuagcUCGAcacguaGCGCGUcgauauggCUCAu -3' miRNA: 3'- -GCUACGCCGCU---------AGCU------CGCGCAa-------GAGU- -5' |
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12344 | 5' | -55.2 | NC_003324.1 | + | 40591 | 0.68 | 0.642685 |
Target: 5'- uCGAUcGUGGCuccAUCGGGCGUGgugcgCUCAg -3' miRNA: 3'- -GCUA-CGCCGc--UAGCUCGCGCaa---GAGU- -5' |
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12344 | 5' | -55.2 | NC_003324.1 | + | 32385 | 0.73 | 0.368723 |
Target: 5'- aGAUGCGGCGccgcgcagaggccGUUGucgccAGCGUGUUCUCu -3' miRNA: 3'- gCUACGCCGC-------------UAGC-----UCGCGCAAGAGu -5' |
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12344 | 5' | -55.2 | NC_003324.1 | + | 29480 | 0.66 | 0.718346 |
Target: 5'- uGGcUGCgGGCGcGUCGGGCGCa-UCUCGg -3' miRNA: 3'- gCU-ACG-CCGC-UAGCUCGCGcaAGAGU- -5' |
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12344 | 5' | -55.2 | NC_003324.1 | + | 27467 | 0.67 | 0.661238 |
Target: 5'- gGAUGCGGCGAUgCGAacuucuccgccgaaGUGcCGUUC-CAu -3' miRNA: 3'- gCUACGCCGCUA-GCU--------------CGC-GCAAGaGU- -5' |
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12344 | 5' | -55.2 | NC_003324.1 | + | 23536 | 0.66 | 0.728906 |
Target: 5'- uCGGUGCcccacagcguGGCGG-CGGGCGCG--CUCGg -3' miRNA: 3'- -GCUACG----------CCGCUaGCUCGCGCaaGAGU- -5' |
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12344 | 5' | -55.2 | NC_003324.1 | + | 22966 | 0.68 | 0.642685 |
Target: 5'- gCGAUGCGaGCGAaCaGGCGCuugcgGUUCUCc -3' miRNA: 3'- -GCUACGC-CGCUaGcUCGCG-----CAAGAGu -5' |
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12344 | 5' | -55.2 | NC_003324.1 | + | 21387 | 0.66 | 0.749729 |
Target: 5'- uCGAucgucUGCcacguccGCGcAUCGAGCGUGUUCUUg -3' miRNA: 3'- -GCU-----ACGc------CGC-UAGCUCGCGCAAGAGu -5' |
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12344 | 5' | -55.2 | NC_003324.1 | + | 21313 | 0.67 | 0.707702 |
Target: 5'- ---cGcCGGCGAuauccUCGAuuucGCGCGUUUUCAg -3' miRNA: 3'- gcuaC-GCCGCU-----AGCU----CGCGCAAGAGU- -5' |
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12344 | 5' | -55.2 | NC_003324.1 | + | 20547 | 1.09 | 0.001175 |
Target: 5'- gCGAUGCGGCGAUCGAGCGCGUUCUCAa -3' miRNA: 3'- -GCUACGCCGCUAGCUCGCGCAAGAGU- -5' |
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12344 | 5' | -55.2 | NC_003324.1 | + | 17792 | 0.69 | 0.588134 |
Target: 5'- uGAUGcCGGCGAUCuGGUaGCGUgcCUCAc -3' miRNA: 3'- gCUAC-GCCGCUAGcUCG-CGCAa-GAGU- -5' |
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12344 | 5' | -55.2 | NC_003324.1 | + | 8814 | 0.69 | 0.577298 |
Target: 5'- uGGUGCGGCcuUCGAGCGUaGUUacgaUCGu -3' miRNA: 3'- gCUACGCCGcuAGCUCGCG-CAAg---AGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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