Results 21 - 28 of 28 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
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Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 12345 | 5' | -61.5 | NC_003324.1 | + | 44595 | 0.68 | 0.317983 |
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Target: 5'- aGAcGCaCCGcaUGcGCGuGCCGUCCGAUCg -3' miRNA: 3'- gCUaCGcGGC--AC-CGC-CGGCAGGCUGG- -5' |
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| 12345 | 5' | -61.5 | NC_003324.1 | + | 21157 | 0.68 | 0.325417 |
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Target: 5'- ---cGCGCCGcGGCGGCCGacaaGGCa -3' miRNA: 3'- gcuaCGCGGCaCCGCCGGCagg-CUGg -5' |
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| 12345 | 5' | -61.5 | NC_003324.1 | + | 13173 | 0.68 | 0.331455 |
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Target: 5'- gCGAggcUGCGUCGgcggcggucggcGGCGGCgGUaCCGGCUg -3' miRNA: 3'- -GCU---ACGCGGCa-----------CCGCCGgCA-GGCUGG- -5' |
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| 12345 | 5' | -61.5 | NC_003324.1 | + | 32470 | 0.67 | 0.389461 |
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Target: 5'- ---cGCGCCGaGGUGGUgcgagaGcCCGACCa -3' miRNA: 3'- gcuaCGCGGCaCCGCCGg-----CaGGCUGG- -5' |
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| 12345 | 5' | -61.5 | NC_003324.1 | + | 33928 | 0.66 | 0.412862 |
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Target: 5'- uCGAUGUggaccucuacgauuGCCGcgaGGCGGCCGUgCaaGCCg -3' miRNA: 3'- -GCUACG--------------CGGCa--CCGCCGGCAgGc-UGG- -5' |
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| 12345 | 5' | -61.5 | NC_003324.1 | + | 54798 | 0.66 | 0.415516 |
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Target: 5'- aGAUGCGCCGau-CGaG-CGUUCGACCg -3' miRNA: 3'- gCUACGCGGCaccGC-CgGCAGGCUGG- -5' |
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| 12345 | 5' | -61.5 | NC_003324.1 | + | 48710 | 0.66 | 0.461218 |
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Target: 5'- uGAUGUugcggcggaGCCGgauGCGGgCG-CCGACCu -3' miRNA: 3'- gCUACG---------CGGCac-CGCCgGCaGGCUGG- -5' |
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| 12345 | 5' | -61.5 | NC_003324.1 | + | 21602 | 1.1 | 0.000263 |
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Target: 5'- uCGAUGCGCCGUGGCGGCCGUCCGACCu -3' miRNA: 3'- -GCUACGCGGCACCGCCGGCAGGCUGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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