Results 21 - 25 of 25 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
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Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 12346 | 3' | -57.7 | NC_003324.1 | + | 38824 | 0.7 | 0.323428 |
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Target: 5'- cGUGCGGGUccAUGGCGuauucGUCGGCg -3' miRNA: 3'- -UACGCCCGa-UACCGCugauaCGGCCG- -5' |
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| 12346 | 3' | -57.7 | NC_003324.1 | + | 37979 | 0.7 | 0.339456 |
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Target: 5'- aAUGUugagGGGUUA-GGCGGCcAUGCCGGa -3' miRNA: 3'- -UACG----CCCGAUaCCGCUGaUACGGCCg -5' |
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| 12346 | 3' | -57.7 | NC_003324.1 | + | 18804 | 0.69 | 0.373211 |
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Target: 5'- -cGCGGGUccAUGGCGcgGCgaagGUCGGCg -3' miRNA: 3'- uaCGCCCGa-UACCGC--UGaua-CGGCCG- -5' |
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| 12346 | 3' | -57.7 | NC_003324.1 | + | 24295 | 0.67 | 0.463976 |
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Target: 5'- -gGCGGaGCU-UGGCGaaggcgucgcggacGCgcaccAUGCCGGCa -3' miRNA: 3'- uaCGCC-CGAuACCGC--------------UGa----UACGGCCG- -5' |
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| 12346 | 3' | -57.7 | NC_003324.1 | + | 44421 | 0.74 | 0.177356 |
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Target: 5'- uGUGCGGGCaccgGGCGccgagacuucggucGCUGaugGCCGGCg -3' miRNA: 3'- -UACGCCCGaua-CCGC--------------UGAUa--CGGCCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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