Results 1 - 20 of 20 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
12348 | 3' | -55.2 | NC_003324.1 | + | 28239 | 0.68 | 0.602641 |
Target: 5'- cGCGUCAUCGAcuGcUGCGaCUgGUGCCGGa -3' miRNA: 3'- -UGUAGUAGCU--CaACGC-GGgCGUGGUC- -5' |
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12348 | 3' | -55.2 | NC_003324.1 | + | 44833 | 0.68 | 0.613666 |
Target: 5'- gGCGUCAUUGGc--GUGCCCGCugCu- -3' miRNA: 3'- -UGUAGUAGCUcaaCGCGGGCGugGuc -5' |
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12348 | 3' | -55.2 | NC_003324.1 | + | 50241 | 0.67 | 0.635755 |
Target: 5'- gACAUgAUCGAGgcGaaaGCCCGCGuCgAGa -3' miRNA: 3'- -UGUAgUAGCUCaaCg--CGGGCGU-GgUC- -5' |
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12348 | 3' | -55.2 | NC_003324.1 | + | 35843 | 0.66 | 0.732701 |
Target: 5'- cCAUCAgggauUCGAccUGCGCCuggcgaacaguggCGCGCCAGu -3' miRNA: 3'- uGUAGU-----AGCUcaACGCGG-------------GCGUGGUC- -5' |
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12348 | 3' | -55.2 | NC_003324.1 | + | 54112 | 0.66 | 0.73376 |
Target: 5'- cGCAUCAccaaccUCGAaaaGUUgGCGCCUGCGCa-- -3' miRNA: 3'- -UGUAGU------AGCU---CAA-CGCGGGCGUGguc -5' |
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12348 | 3' | -55.2 | NC_003324.1 | + | 32548 | 0.68 | 0.580676 |
Target: 5'- aGCuuuUCGUCuccGUUGCGCUCGUGCCAu -3' miRNA: 3'- -UGu--AGUAGcu-CAACGCGGGCGUGGUc -5' |
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12348 | 3' | -55.2 | NC_003324.1 | + | 23315 | 1.09 | 0.00101 |
Target: 5'- gACAUCAUCGAGUUGCGCCCGCACCAGa -3' miRNA: 3'- -UGUAGUAGCUCAACGCGGGCGUGGUC- -5' |
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12348 | 3' | -55.2 | NC_003324.1 | + | 15735 | 0.74 | 0.268788 |
Target: 5'- cGCGUCAUCGAGc-GCGCCgGgGCCGu -3' miRNA: 3'- -UGUAGUAGCUCaaCGCGGgCgUGGUc -5' |
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12348 | 3' | -55.2 | NC_003324.1 | + | 1621 | 0.73 | 0.328385 |
Target: 5'- --uUCAUCGGGgccauaGCGCCgCGCACCAc -3' miRNA: 3'- uguAGUAGCUCaa----CGCGG-GCGUGGUc -5' |
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12348 | 3' | -55.2 | NC_003324.1 | + | 39870 | 0.72 | 0.379152 |
Target: 5'- uCGUCAUCGAGggcgagGUGCaCCGUGCCGa -3' miRNA: 3'- uGUAGUAGCUCaa----CGCG-GGCGUGGUc -5' |
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12348 | 3' | -55.2 | NC_003324.1 | + | 49718 | 0.7 | 0.464525 |
Target: 5'- aGCGUCGUCGaAGUUGCGaUCGUuggcgccuGCCAGa -3' miRNA: 3'- -UGUAGUAGC-UCAACGCgGGCG--------UGGUC- -5' |
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12348 | 3' | -55.2 | NC_003324.1 | + | 23632 | 0.7 | 0.495148 |
Target: 5'- cCGUCGcCGAGc-GCGCCCGcCGCCAc -3' miRNA: 3'- uGUAGUaGCUCaaCGCGGGC-GUGGUc -5' |
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12348 | 3' | -55.2 | NC_003324.1 | + | 31126 | 0.68 | 0.591641 |
Target: 5'- cGCGUCAgcagGAGUUGaaCGCcgaCCGCGCCGGu -3' miRNA: 3'- -UGUAGUag--CUCAAC--GCG---GGCGUGGUC- -5' |
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12348 | 3' | -55.2 | NC_003324.1 | + | 26646 | 0.67 | 0.624707 |
Target: 5'- -gAUCucggCGAGgaugUGCGUCCGUACCuGa -3' miRNA: 3'- ugUAGua--GCUCa---ACGCGGGCGUGGuC- -5' |
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12348 | 3' | -55.2 | NC_003324.1 | + | 39839 | 0.67 | 0.624707 |
Target: 5'- -gAUCAUCGAGcacgUGcCGCCgGaCACCAa -3' miRNA: 3'- ugUAGUAGCUCa---AC-GCGGgC-GUGGUc -5' |
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12348 | 3' | -55.2 | NC_003324.1 | + | 26699 | 0.67 | 0.624707 |
Target: 5'- ---aUAUCGAGaaggGUGCCCGUACCu- -3' miRNA: 3'- uguaGUAGCUCaa--CGCGGGCGUGGuc -5' |
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12348 | 3' | -55.2 | NC_003324.1 | + | 20830 | 0.67 | 0.635755 |
Target: 5'- cGCAUggccgaCAUCGAGaagcagGCGCCUGCGCUc- -3' miRNA: 3'- -UGUA------GUAGCUCaa----CGCGGGCGUGGuc -5' |
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12348 | 3' | -55.2 | NC_003324.1 | + | 47778 | 0.66 | 0.688554 |
Target: 5'- cAUGUCGUCGAGgaggccgcgaagcGCGCCgacggaacuguccagCGCACCGGc -3' miRNA: 3'- -UGUAGUAGCUCaa-----------CGCGG---------------GCGUGGUC- -5' |
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12348 | 3' | -55.2 | NC_003324.1 | + | 29569 | 0.66 | 0.690736 |
Target: 5'- -gGUUAcCGAGaUGCGCCCGacgcgcccgcaGCCAGg -3' miRNA: 3'- ugUAGUaGCUCaACGCGGGCg----------UGGUC- -5' |
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12348 | 3' | -55.2 | NC_003324.1 | + | 32916 | 0.66 | 0.73376 |
Target: 5'- cGCGUCAguggcgaugaUCGAGgcgGCuGCCCGCcaugcGCCGa -3' miRNA: 3'- -UGUAGU----------AGCUCaa-CG-CGGGCG-----UGGUc -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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