miRNA display CGI


Results 1 - 5 of 5 are showing below:
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ID Location Perfect MFE.* RefSeq Acc. strand Start Position R_P_ratio# P value
Predicted miRNA align pattern
1235 3' -55.8 NC_001271.1 + 22137 0.67 0.541809
Target:  5'- aGCCGACCAC--UGGCGACCugacugaccCGGUugaGCu -3'
miRNA:   3'- -UGGCUGGUGuaACCGUUGGc--------GUCG---CG- -5'
1235 3' -55.8 NC_001271.1 + 1192 0.7 0.336302
Target:  5'- gACCGgguGCUAUcgUgaGGCAAUCGCaAGCGCa -3'
miRNA:   3'- -UGGC---UGGUGuaA--CCGUUGGCG-UCGCG- -5'
1235 3' -55.8 NC_001271.1 + 13677 0.71 0.319919
Target:  5'- cACCGACCACcUUGucaGCAGCCauguCAGgGCa -3'
miRNA:   3'- -UGGCUGGUGuAAC---CGUUGGc---GUCgCG- -5'
1235 3' -55.8 NC_001271.1 + 18995 0.72 0.28161
Target:  5'- uACCGACUACAcgacuccgUGGCAgGCUGUAgguGCGCg -3'
miRNA:   3'- -UGGCUGGUGUa-------ACCGU-UGGCGU---CGCG- -5'
1235 3' -55.8 NC_001271.1 + 32857 1.11 0.000401
Target:  5'- uACCGACCACAUUGGCAACCGCAGCGCu -3'
miRNA:   3'- -UGGCUGGUGUAACCGUUGGCGUCGCG- -5'
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Note:
      When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.

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TDL, Institute of Biomedical Science, Academia Sinica, Taipei, Taiwan.
Copyright © 2007 TDL. All rights reserved.