Results 1 - 5 of 5 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
1235 | 3' | -55.8 | NC_001271.1 | + | 22137 | 0.67 | 0.541809 |
Target: 5'- aGCCGACCAC--UGGCGACCugacugaccCGGUugaGCu -3' miRNA: 3'- -UGGCUGGUGuaACCGUUGGc--------GUCG---CG- -5' |
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1235 | 3' | -55.8 | NC_001271.1 | + | 1192 | 0.7 | 0.336302 |
Target: 5'- gACCGgguGCUAUcgUgaGGCAAUCGCaAGCGCa -3' miRNA: 3'- -UGGC---UGGUGuaA--CCGUUGGCG-UCGCG- -5' |
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1235 | 3' | -55.8 | NC_001271.1 | + | 13677 | 0.71 | 0.319919 |
Target: 5'- cACCGACCACcUUGucaGCAGCCauguCAGgGCa -3' miRNA: 3'- -UGGCUGGUGuAAC---CGUUGGc---GUCgCG- -5' |
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1235 | 3' | -55.8 | NC_001271.1 | + | 18995 | 0.72 | 0.28161 |
Target: 5'- uACCGACUACAcgacuccgUGGCAgGCUGUAgguGCGCg -3' miRNA: 3'- -UGGCUGGUGUa-------ACCGU-UGGCGU---CGCG- -5' |
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1235 | 3' | -55.8 | NC_001271.1 | + | 32857 | 1.11 | 0.000401 |
Target: 5'- uACCGACCACAUUGGCAACCGCAGCGCu -3' miRNA: 3'- -UGGCUGGUGUAACCGUUGGCGUCGCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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