miRNA display CGI


Results 1 - 11 of 11 are showing below:
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ID Location Perfect MFE.* RefSeq Acc. strand Start Position R_P_ratio# P value
Predicted miRNA align pattern
12350 5' -53.5 NC_003324.1 + 9640 0.66 0.796812
Target:  5'- gGCCAacACCg--CCGGCAACGcCAAc -3'
miRNA:   3'- -UGGUgaUGGacaGGCCGUUGCaGUUc -5'
12350 5' -53.5 NC_003324.1 + 12281 0.66 0.796812
Target:  5'- cCCGCgagACC-GUCCGGCGGC-UCGu- -3'
miRNA:   3'- uGGUGa--UGGaCAGGCCGUUGcAGUuc -5'
12350 5' -53.5 NC_003324.1 + 31005 0.66 0.786924
Target:  5'- cACCggACgcauCCUGaaaUCCGGCGugGUCGGa -3'
miRNA:   3'- -UGG--UGau--GGAC---AGGCCGUugCAGUUc -5'
12350 5' -53.5 NC_003324.1 + 20963 0.67 0.780912
Target:  5'- uAUCAC--CCUGUCCGGCgcccgugauuaucacGugcACGUCGAGg -3'
miRNA:   3'- -UGGUGauGGACAGGCCG---------------U---UGCAGUUC- -5'
12350 5' -53.5 NC_003324.1 + 28535 0.67 0.745839
Target:  5'- gACCACUgagGCCUG-CCgcgcucaacauGGCGGCGUUAGc -3'
miRNA:   3'- -UGGUGA---UGGACaGG-----------CCGUUGCAGUUc -5'
12350 5' -53.5 NC_003324.1 + 47856 0.67 0.735244
Target:  5'- uGCCGCUgaaaccguACCUGaCCGGC---GUCAGGa -3'
miRNA:   3'- -UGGUGA--------UGGACaGGCCGuugCAGUUC- -5'
12350 5' -53.5 NC_003324.1 + 43696 0.68 0.713746
Target:  5'- cCgGCUugCUGUCCGaGCA--GUCGAGc -3'
miRNA:   3'- uGgUGAugGACAGGC-CGUugCAGUUC- -5'
12350 5' -53.5 NC_003324.1 + 31427 0.68 0.680911
Target:  5'- cCCACUuACC-GUCCGGCAACugccauGUCGu- -3'
miRNA:   3'- uGGUGA-UGGaCAGGCCGUUG------CAGUuc -5'
12350 5' -53.5 NC_003324.1 + 51092 0.68 0.680911
Target:  5'- gGCCAUUucgucgGCCUucGUCCGaGCcuCGUCGAGg -3'
miRNA:   3'- -UGGUGA------UGGA--CAGGC-CGuuGCAGUUC- -5'
12350 5' -53.5 NC_003324.1 + 11144 0.68 0.668748
Target:  5'- uCCGCUucGCCgGUCUGGCGgccggcucgcacaGCGUUGAGg -3'
miRNA:   3'- uGGUGA--UGGaCAGGCCGU-------------UGCAGUUC- -5'
12350 5' -53.5 NC_003324.1 + 23896 1.09 0.001508
Target:  5'- gACCACUACCUGUCCGGCAACGUCAAGa -3'
miRNA:   3'- -UGGUGAUGGACAGGCCGUUGCAGUUC- -5' 
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Note:
      When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.

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TDL, Institute of Biomedical Science, Academia Sinica, Taipei, Taiwan.
Copyright © 2007 TDL. All rights reserved.