Results 1 - 17 of 17 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
12351 | 5' | -58.9 | NC_003324.1 | + | 36633 | 0.66 | 0.435444 |
Target: 5'- gUCUCgAuGCGCAUGUAcGUcGCCCUAu -3' miRNA: 3'- aAGAGgUuCGCGUGCGU-CGuCGGGAU- -5' |
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12351 | 5' | -58.9 | NC_003324.1 | + | 24098 | 1.04 | 0.000731 |
Target: 5'- cUUCUCCAAGCGCACGCAGCAGCCCUAc -3' miRNA: 3'- -AAGAGGUUCGCGUGCGUCGUCGGGAU- -5' |
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12351 | 5' | -58.9 | NC_003324.1 | + | 31204 | 0.7 | 0.235943 |
Target: 5'- ---gCCAGGCGCAagaacaaGCAGCAGCCg-- -3' miRNA: 3'- aagaGGUUCGCGUg------CGUCGUCGGgau -5' |
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12351 | 5' | -58.9 | NC_003324.1 | + | 55136 | 0.7 | 0.255156 |
Target: 5'- -gCUUCu-GCGCcguCGCGGCAGCCCa- -3' miRNA: 3'- aaGAGGuuCGCGu--GCGUCGUCGGGau -5' |
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12351 | 5' | -58.9 | NC_003324.1 | + | 8280 | 0.69 | 0.273529 |
Target: 5'- gUCgaagugCCAGGCGCGCGCcgccgGGCagugagcagggacuGGCCCUGa -3' miRNA: 3'- aAGa-----GGUUCGCGUGCG-----UCG--------------UCGGGAU- -5' |
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12351 | 5' | -58.9 | NC_003324.1 | + | 22300 | 0.69 | 0.282746 |
Target: 5'- -gCUCCcgaAGGUGCGCgGCGGCaAGCCCa- -3' miRNA: 3'- aaGAGG---UUCGCGUG-CGUCG-UCGGGau -5' |
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12351 | 5' | -58.9 | NC_003324.1 | + | 31630 | 0.69 | 0.282746 |
Target: 5'- aUCgagaCCGcgGGCGCGCGUGGCGGCCa-- -3' miRNA: 3'- aAGa---GGU--UCGCGUGCGUCGUCGGgau -5' |
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12351 | 5' | -58.9 | NC_003324.1 | + | 28165 | 0.69 | 0.297405 |
Target: 5'- -gCUCCGAGCGCAaagGCAcgAGCCCa- -3' miRNA: 3'- aaGAGGUUCGCGUg--CGUcgUCGGGau -5' |
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12351 | 5' | -58.9 | NC_003324.1 | + | 38522 | 0.67 | 0.361876 |
Target: 5'- -aCUUCGAGUGCA-GCAGCgAGCCgUAu -3' miRNA: 3'- aaGAGGUUCGCGUgCGUCG-UCGGgAU- -5' |
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12351 | 5' | -58.9 | NC_003324.1 | + | 32226 | 0.66 | 0.435444 |
Target: 5'- -aUUCCAAGC-C-CGgGGCGGCCCg- -3' miRNA: 3'- aaGAGGUUCGcGuGCgUCGUCGGGau -5' |
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12351 | 5' | -58.9 | NC_003324.1 | + | 14329 | 0.66 | 0.455147 |
Target: 5'- cUUUUCguAGCGguCGCGGuCAGCCUUc -3' miRNA: 3'- -AAGAGguUCGCguGCGUC-GUCGGGAu -5' |
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12351 | 5' | -58.9 | NC_003324.1 | + | 23826 | 0.66 | 0.465178 |
Target: 5'- aUUUCC-GGCGCaACGUcGCAGCUCUu -3' miRNA: 3'- aAGAGGuUCGCG-UGCGuCGUCGGGAu -5' |
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12351 | 5' | -58.9 | NC_003324.1 | + | 4565 | 0.7 | 0.235943 |
Target: 5'- ---aUCGAGCGCGCGCAGguccaGGCCCg- -3' miRNA: 3'- aagaGGUUCGCGUGCGUCg----UCGGGau -5' |
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12351 | 5' | -58.9 | NC_003324.1 | + | 44334 | 0.68 | 0.344884 |
Target: 5'- cUCUgCAgccacGGCGCGCGCuGC-GCCCUu -3' miRNA: 3'- aAGAgGU-----UCGCGUGCGuCGuCGGGAu -5' |
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12351 | 5' | -58.9 | NC_003324.1 | + | 44195 | 0.68 | 0.335786 |
Target: 5'- -cCUCCAAGCGUucgAUGCGGUggaugaaGGCCUUGa -3' miRNA: 3'- aaGAGGUUCGCG---UGCGUCG-------UCGGGAU- -5' |
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12351 | 5' | -58.9 | NC_003324.1 | + | 48364 | 0.69 | 0.275634 |
Target: 5'- -aCUCCAAGCcgGCuuCGCAGUuGCCCUu -3' miRNA: 3'- aaGAGGUUCG--CGu-GCGUCGuCGGGAu -5' |
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12351 | 5' | -58.9 | NC_003324.1 | + | 46400 | 0.7 | 0.248613 |
Target: 5'- -cCUUgAGGCGCuuGCGGUAGUCCUGc -3' miRNA: 3'- aaGAGgUUCGCGugCGUCGUCGGGAU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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