Results 1 - 7 of 7 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 12352 | 5' | -54.3 | NC_003324.1 | + | 27071 | 0.67 | 0.73897 |
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Target: 5'- gGGCAGAGACcGgcggcaaCACCCUCaUCGAGGg -3' miRNA: 3'- -CUGUCUUUGaCa------GUGGGAGgGGCUCU- -5' |
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| 12352 | 5' | -54.3 | NC_003324.1 | + | 36375 | 0.68 | 0.674116 |
|
Target: 5'- uGGCGGAAGaaaUCGCCCgCCCCGAu- -3' miRNA: 3'- -CUGUCUUUgacAGUGGGaGGGGCUcu -5' |
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| 12352 | 5' | -54.3 | NC_003324.1 | + | 56656 | 0.69 | 0.596759 |
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Target: 5'- -cCAGAGACcucaccucacgGUCACgUUCCCUGAGAa -3' miRNA: 3'- cuGUCUUUGa----------CAGUGgGAGGGGCUCU- -5' |
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| 12352 | 5' | -54.3 | NC_003324.1 | + | 24484 | 0.7 | 0.542204 |
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Target: 5'- uGACAGuuuCUGUCACCgucugcgCCCCGGGc -3' miRNA: 3'- -CUGUCuuuGACAGUGGga-----GGGGCUCu -5' |
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| 12352 | 5' | -54.3 | NC_003324.1 | + | 37565 | 0.7 | 0.542204 |
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Target: 5'- cGCaAGAAGCgcuaccaGUCGCCCaUCCCgGAGAc -3' miRNA: 3'- cUG-UCUUUGa------CAGUGGG-AGGGgCUCU- -5' |
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| 12352 | 5' | -54.3 | NC_003324.1 | + | 26729 | 0.7 | 0.531476 |
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Target: 5'- uGCAGGcAGgUGUCACCCUCgCCGGc- -3' miRNA: 3'- cUGUCU-UUgACAGUGGGAGgGGCUcu -5' |
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| 12352 | 5' | -54.3 | NC_003324.1 | + | 24554 | 1.09 | 0.001323 |
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Target: 5'- uGACAGAAACUGUCACCCUCCCCGAGAa -3' miRNA: 3'- -CUGUCUUUGACAGUGGGAGGGGCUCU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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