Results 1 - 19 of 19 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
12353 | 5' | -55 | NC_003324.1 | + | 49202 | 0.66 | 0.724542 |
Target: 5'- uCGAUCUuCCUGCCG-ACGAUccgaacGCCUg -3' miRNA: 3'- cGCUAGGcGGACGGCuUGUUA------CGGAa -5' |
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12353 | 5' | -55 | NC_003324.1 | + | 49704 | 0.66 | 0.724542 |
Target: 5'- uGCGAUCguuggCGCCUGCCaGAaugACGGUaucGCCa- -3' miRNA: 3'- -CGCUAG-----GCGGACGG-CU---UGUUA---CGGaa -5' |
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12353 | 5' | -55 | NC_003324.1 | + | 14572 | 0.66 | 0.702868 |
Target: 5'- -gGGUCgCgGCCUGCCGGcGCAAgcgGCCg- -3' miRNA: 3'- cgCUAG-G-CGGACGGCU-UGUUa--CGGaa -5' |
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12353 | 5' | -55 | NC_003324.1 | + | 42169 | 0.66 | 0.702868 |
Target: 5'- cGCGAUCguaCUUGCCGAACGAcgGCaCUg -3' miRNA: 3'- -CGCUAGgc-GGACGGCUUGUUa-CG-GAa -5' |
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12353 | 5' | -55 | NC_003324.1 | + | 41192 | 0.66 | 0.679808 |
Target: 5'- -aGGUCCGCUUGCCagcggauGGACA-UGCUUUc -3' miRNA: 3'- cgCUAGGCGGACGG-------CUUGUuACGGAA- -5' |
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12353 | 5' | -55 | NC_003324.1 | + | 4993 | 0.66 | 0.679808 |
Target: 5'- aGCGGaagCCGCCUGCgGAgccgaaaGCGAUaacGCCa- -3' miRNA: 3'- -CGCUa--GGCGGACGgCU-------UGUUA---CGGaa -5' |
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12353 | 5' | -55 | NC_003324.1 | + | 5537 | 0.67 | 0.66764 |
Target: 5'- gGCGAcgCCGCCUGCCuacccagcguCGAUGCg-- -3' miRNA: 3'- -CGCUa-GGCGGACGGcuu-------GUUACGgaa -5' |
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12353 | 5' | -55 | NC_003324.1 | + | 35547 | 0.67 | 0.647648 |
Target: 5'- uGUGAUCUGCgaGCgGAucuGCAagGCCUg -3' miRNA: 3'- -CGCUAGGCGgaCGgCU---UGUuaCGGAa -5' |
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12353 | 5' | -55 | NC_003324.1 | + | 50918 | 0.67 | 0.636516 |
Target: 5'- gGCGAaCCGCCUGCa--GCGAUGUg-- -3' miRNA: 3'- -CGCUaGGCGGACGgcuUGUUACGgaa -5' |
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12353 | 5' | -55 | NC_003324.1 | + | 30286 | 0.67 | 0.636516 |
Target: 5'- aCGGUCgcccaaGCC-GCCGAAC-AUGCCUg -3' miRNA: 3'- cGCUAGg-----CGGaCGGCUUGuUACGGAa -5' |
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12353 | 5' | -55 | NC_003324.1 | + | 17168 | 0.67 | 0.629834 |
Target: 5'- aGCGAUUgacgaggcgcuggcgCGUCUGUCGGACGcgGCUUUc -3' miRNA: 3'- -CGCUAG---------------GCGGACGGCUUGUuaCGGAA- -5' |
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12353 | 5' | -55 | NC_003324.1 | + | 43100 | 0.67 | 0.62538 |
Target: 5'- cGCcAUCCGCCaGCgGGcAUAAUGCCa- -3' miRNA: 3'- -CGcUAGGCGGaCGgCU-UGUUACGGaa -5' |
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12353 | 5' | -55 | NC_003324.1 | + | 28640 | 0.68 | 0.603137 |
Target: 5'- uGCGggCCGCCuUGUCGcGCGucgugGUGCCa- -3' miRNA: 3'- -CGCuaGGCGG-ACGGCuUGU-----UACGGaa -5' |
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12353 | 5' | -55 | NC_003324.1 | + | 40154 | 0.68 | 0.596481 |
Target: 5'- gGCGAuguccuugUCCGacaCCUucgacaggaacaucaGCCGAGCGAUGCCg- -3' miRNA: 3'- -CGCU--------AGGC---GGA---------------CGGCUUGUUACGGaa -5' |
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12353 | 5' | -55 | NC_003324.1 | + | 1585 | 0.68 | 0.580996 |
Target: 5'- -gGAUCCucgcugGCCcgGCCGGaaGCAAUGCCa- -3' miRNA: 3'- cgCUAGG------CGGa-CGGCU--UGUUACGGaa -5' |
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12353 | 5' | -55 | NC_003324.1 | + | 42300 | 0.69 | 0.537299 |
Target: 5'- aGUGcgCCGCCUGCC--ACG-UGCCg- -3' miRNA: 3'- -CGCuaGGCGGACGGcuUGUuACGGaa -5' |
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12353 | 5' | -55 | NC_003324.1 | + | 16248 | 0.73 | 0.303426 |
Target: 5'- uGCGGUgcCCGCCucggUGCC-AGCGAUGCCUg -3' miRNA: 3'- -CGCUA--GGCGG----ACGGcUUGUUACGGAa -5' |
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12353 | 5' | -55 | NC_003324.1 | + | 4378 | 0.74 | 0.260313 |
Target: 5'- cGCGGUgCGCUUGCgGGACAucuUGCCa- -3' miRNA: 3'- -CGCUAgGCGGACGgCUUGUu--ACGGaa -5' |
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12353 | 5' | -55 | NC_003324.1 | + | 24895 | 1.07 | 0.001296 |
Target: 5'- cGCGAUCCGCCUGCCGAACAAUGCCUUc -3' miRNA: 3'- -CGCUAGGCGGACGGCUUGUUACGGAA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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