Results 1 - 19 of 19 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
12357 | 3' | -58.1 | NC_003324.1 | + | 35229 | 0.66 | 0.577014 |
Target: 5'- -cGGCGcCGAaggUGACGaAGGCGCUgAGg -3' miRNA: 3'- uaCCGCcGCUa--GCUGC-UCCGUGAgUC- -5' |
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12357 | 3' | -58.1 | NC_003324.1 | + | 22222 | 0.66 | 0.534526 |
Target: 5'- -cGGCgaGGCGGaCGACGAcGGCAauCUCAu -3' miRNA: 3'- uaCCG--CCGCUaGCUGCU-CCGU--GAGUc -5' |
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12357 | 3' | -58.1 | NC_003324.1 | + | 52222 | 0.67 | 0.503398 |
Target: 5'- cAUGGCGG-GuUCGGCGGGcuauCACUCGGc -3' miRNA: 3'- -UACCGCCgCuAGCUGCUCc---GUGAGUC- -5' |
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12357 | 3' | -58.1 | NC_003324.1 | + | 7860 | 0.67 | 0.493196 |
Target: 5'- -cGGCGGCaaagcccguugcGAUCGcgccucccuGCGAGGCGugcuCUCGGa -3' miRNA: 3'- uaCCGCCG------------CUAGC---------UGCUCCGU----GAGUC- -5' |
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12357 | 3' | -58.1 | NC_003324.1 | + | 4504 | 0.67 | 0.473083 |
Target: 5'- -cGGCGGCGAcgCGACuacuaAGGC-CUCGa -3' miRNA: 3'- uaCCGCCGCUa-GCUGc----UCCGuGAGUc -5' |
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12357 | 3' | -58.1 | NC_003324.1 | + | 47709 | 0.67 | 0.470102 |
Target: 5'- gAUGGCGGCccggucgccgucauGcgCGGCGuGGUaaaGCUCGGa -3' miRNA: 3'- -UACCGCCG--------------CuaGCUGCuCCG---UGAGUC- -5' |
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12357 | 3' | -58.1 | NC_003324.1 | + | 45997 | 0.67 | 0.463183 |
Target: 5'- -aGGCcuCGAuuUCGGCGAGGUACUCu- -3' miRNA: 3'- uaCCGccGCU--AGCUGCUCCGUGAGuc -5' |
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12357 | 3' | -58.1 | NC_003324.1 | + | 21893 | 0.68 | 0.443716 |
Target: 5'- -aGGCGGaCGAcggCGACGAGaaagcacaggaaGCGCUCGa -3' miRNA: 3'- uaCCGCC-GCUa--GCUGCUC------------CGUGAGUc -5' |
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12357 | 3' | -58.1 | NC_003324.1 | + | 25325 | 0.68 | 0.434156 |
Target: 5'- cUGGUGGaCGAuUCGGCaGAGGCguAUUCAGc -3' miRNA: 3'- uACCGCC-GCU-AGCUG-CUCCG--UGAGUC- -5' |
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12357 | 3' | -58.1 | NC_003324.1 | + | 20599 | 0.68 | 0.424717 |
Target: 5'- -gGGCGcuuGCGGUCGGCG-GGCACg--- -3' miRNA: 3'- uaCCGC---CGCUAGCUGCuCCGUGaguc -5' |
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12357 | 3' | -58.1 | NC_003324.1 | + | 7102 | 0.68 | 0.415402 |
Target: 5'- uUGGC-GCGAaagCGACGAGGuCACgaUCAGu -3' miRNA: 3'- uACCGcCGCUa--GCUGCUCC-GUG--AGUC- -5' |
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12357 | 3' | -58.1 | NC_003324.1 | + | 22961 | 0.69 | 0.406213 |
Target: 5'- -cGGUGGCGAUgCGAgCGAacaGGCGCUUg- -3' miRNA: 3'- uaCCGCCGCUA-GCU-GCU---CCGUGAGuc -5' |
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12357 | 3' | -58.1 | NC_003324.1 | + | 6220 | 0.69 | 0.38557 |
Target: 5'- -aGGCGGCGcgggucaguccgcuAUCGGCGuAGGCGCg--- -3' miRNA: 3'- uaCCGCCGC--------------UAGCUGC-UCCGUGaguc -5' |
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12357 | 3' | -58.1 | NC_003324.1 | + | 23550 | 0.72 | 0.264045 |
Target: 5'- cGUGGCGGCGGgcgcgcUCGGCGAcGGUACa--- -3' miRNA: 3'- -UACCGCCGCU------AGCUGCU-CCGUGaguc -5' |
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12357 | 3' | -58.1 | NC_003324.1 | + | 4600 | 0.73 | 0.226537 |
Target: 5'- -cGGUGGCG-UCGAuCGAGGC-CUUAGu -3' miRNA: 3'- uaCCGCCGCuAGCU-GCUCCGuGAGUC- -5' |
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12357 | 3' | -58.1 | NC_003324.1 | + | 12249 | 0.74 | 0.183643 |
Target: 5'- gAUGGCGGCGuUCGuuG-GGCGCUCGa -3' miRNA: 3'- -UACCGCCGCuAGCugCuCCGUGAGUc -5' |
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12357 | 3' | -58.1 | NC_003324.1 | + | 13161 | 0.76 | 0.129165 |
Target: 5'- -cGGCGGCGGUCGGCGGcggcGGUAC-CGGc -3' miRNA: 3'- uaCCGCCGCUAGCUGCU----CCGUGaGUC- -5' |
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12357 | 3' | -58.1 | NC_003324.1 | + | 17160 | 0.77 | 0.109417 |
Target: 5'- cAUGGCccaGCGAUUGACGAGGCGCUg-- -3' miRNA: 3'- -UACCGc--CGCUAGCUGCUCCGUGAguc -5' |
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12357 | 3' | -58.1 | NC_003324.1 | + | 26923 | 1.06 | 0.00092 |
Target: 5'- cAUGGCGGCGAUCGACGAGGCACUCAGc -3' miRNA: 3'- -UACCGCCGCUAGCUGCUCCGUGAGUC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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