Results 1 - 4 of 4 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
1236 | 3' | -50.2 | NC_001271.1 | + | 3450 | 0.66 | 0.889312 |
Target: 5'- aGGCga--GCGCCgcuuccucaAGAUGCUUGAGCGu -3' miRNA: 3'- -UUGaaugCGCGG---------UCUAUGAGCUUGCc -5' |
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1236 | 3' | -50.2 | NC_001271.1 | + | 33963 | 0.66 | 0.864829 |
Target: 5'- uGACUUACcCGCUGGAUgGCUCcacuACGGa -3' miRNA: 3'- -UUGAAUGcGCGGUCUA-UGAGcu--UGCC- -5' |
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1236 | 3' | -50.2 | NC_001271.1 | + | 18108 | 0.68 | 0.788312 |
Target: 5'- cGC-UACGCGCUgcuGGAUACUCuGAgucuuucauuGCGGg -3' miRNA: 3'- uUGaAUGCGCGG---UCUAUGAG-CU----------UGCC- -5' |
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1236 | 3' | -50.2 | NC_001271.1 | + | 34069 | 1.09 | 0.002113 |
Target: 5'- aAACUUACGCGCCAGAUACUCGAACGGa -3' miRNA: 3'- -UUGAAUGCGCGGUCUAUGAGCUUGCC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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