Results 1 - 15 of 15 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
12361 | 3' | -58 | NC_003324.1 | + | 5506 | 0.66 | 0.586623 |
Target: 5'- cGGAGAaGaacUCGGCGCCGcAgGUGCGGg -3' miRNA: 3'- -CCUCUaCa--GGCCGCGGCuUgCGCGUU- -5' |
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12361 | 3' | -58 | NC_003324.1 | + | 57035 | 0.66 | 0.575914 |
Target: 5'- uGGGGAg---CGGCGgCGAGgGCGCGGa -3' miRNA: 3'- -CCUCUacagGCCGCgGCUUgCGCGUU- -5' |
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12361 | 3' | -58 | NC_003324.1 | + | 48693 | 0.66 | 0.575914 |
Target: 5'- -cGGAUG-CgGGCGCCGAccuGCGUGuCGAu -3' miRNA: 3'- ccUCUACaGgCCGCGGCU---UGCGC-GUU- -5' |
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12361 | 3' | -58 | NC_003324.1 | + | 26411 | 0.66 | 0.575914 |
Target: 5'- cGGGAUGcucgaaCCGGCGCUGAAagaaGcCGCGGa -3' miRNA: 3'- cCUCUACa-----GGCCGCGGCUUg---C-GCGUU- -5' |
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12361 | 3' | -58 | NC_003324.1 | + | 20631 | 0.66 | 0.565249 |
Target: 5'- aGAGAcccgauccgacUGUugCCGGCGCUGAugGgCGCu- -3' miRNA: 3'- cCUCU-----------ACA--GGCCGCGGCUugC-GCGuu -5' |
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12361 | 3' | -58 | NC_003324.1 | + | 18899 | 0.66 | 0.554637 |
Target: 5'- uGGAGA---CUGGCGCCGAccuucgcCGCGCc- -3' miRNA: 3'- -CCUCUacaGGCCGCGGCUu------GCGCGuu -5' |
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12361 | 3' | -58 | NC_003324.1 | + | 20956 | 0.66 | 0.544084 |
Target: 5'- ----cUGUCCGGCGCCcguGAuuaucACGUGCAc -3' miRNA: 3'- ccucuACAGGCCGCGG---CU-----UGCGCGUu -5' |
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12361 | 3' | -58 | NC_003324.1 | + | 39985 | 0.67 | 0.502599 |
Target: 5'- -----aGUCCGucGCGCCGAGCGUgGCGAg -3' miRNA: 3'- ccucuaCAGGC--CGCGGCUUGCG-CGUU- -5' |
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12361 | 3' | -58 | NC_003324.1 | + | 23374 | 0.67 | 0.502599 |
Target: 5'- -cGGAUGUCCuGCGCagcuauGAcgGCGCGCAu -3' miRNA: 3'- ccUCUACAGGcCGCGg-----CU--UGCGCGUu -5' |
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12361 | 3' | -58 | NC_003324.1 | + | 17551 | 0.68 | 0.443168 |
Target: 5'- cGGAaccaaagGUCCGGCauGCCGA-UGCGCGAc -3' miRNA: 3'- -CCUcua----CAGGCCG--CGGCUuGCGCGUU- -5' |
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12361 | 3' | -58 | NC_003324.1 | + | 45847 | 0.68 | 0.452804 |
Target: 5'- uGAGGUGgaaaCGGCGCgGGGCGCcugauGCGAg -3' miRNA: 3'- cCUCUACag--GCCGCGgCUUGCG-----CGUU- -5' |
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12361 | 3' | -58 | NC_003324.1 | + | 32809 | 0.68 | 0.452804 |
Target: 5'- cGAGcUGUUCGGCGCauGGCGgGCAGc -3' miRNA: 3'- cCUCuACAGGCCGCGgcUUGCgCGUU- -5' |
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12361 | 3' | -58 | NC_003324.1 | + | 38005 | 0.7 | 0.362023 |
Target: 5'- cGGAGcgGUUgCGGCGCacgGGGCGCGuCAAc -3' miRNA: 3'- -CCUCuaCAG-GCCGCGg--CUUGCGC-GUU- -5' |
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12361 | 3' | -58 | NC_003324.1 | + | 23640 | 0.74 | 0.183981 |
Target: 5'- -uGGAUGUaCCGuCGCCGAGCGCGCc- -3' miRNA: 3'- ccUCUACA-GGCcGCGGCUUGCGCGuu -5' |
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12361 | 3' | -58 | NC_003324.1 | + | 28372 | 1.08 | 0.000623 |
Target: 5'- cGGAGAUGUCCGGCGCCGAACGCGCAAg -3' miRNA: 3'- -CCUCUACAGGCCGCGGCUUGCGCGUU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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