Results 1 - 20 of 22 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
12361 | 5' | -58.6 | NC_003324.1 | + | 11148 | 0.76 | 0.117118 |
Target: 5'- cUUCGCCGGUCUGGCgGCCGGcUCGCAc -3' miRNA: 3'- aAGGUGGCUAGGCCG-UGGUC-AGCGUc -5' |
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12361 | 5' | -58.6 | NC_003324.1 | + | 24575 | 0.66 | 0.53835 |
Target: 5'- -gUCACCGcugCgGGCgACCAG-CGCAGg -3' miRNA: 3'- aaGGUGGCua-GgCCG-UGGUCaGCGUC- -5' |
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12361 | 5' | -58.6 | NC_003324.1 | + | 7693 | 0.66 | 0.517487 |
Target: 5'- -aCCGCCGAccCCGGaaaCACCGG-CGCGa -3' miRNA: 3'- aaGGUGGCUa-GGCC---GUGGUCaGCGUc -5' |
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12361 | 5' | -58.6 | NC_003324.1 | + | 1516 | 0.66 | 0.517487 |
Target: 5'- -gCCAgCGAggaUCCGGCGgCAGUggugCGCGGc -3' miRNA: 3'- aaGGUgGCU---AGGCCGUgGUCA----GCGUC- -5' |
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12361 | 5' | -58.6 | NC_003324.1 | + | 18153 | 0.67 | 0.486827 |
Target: 5'- gUUUCGCCGAcaCCGGCAa-GGUCGUGGc -3' miRNA: 3'- -AAGGUGGCUa-GGCCGUggUCAGCGUC- -5' |
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12361 | 5' | -58.6 | NC_003324.1 | + | 43396 | 0.67 | 0.476797 |
Target: 5'- -aCCGCCGAUCUGaC-CgCAGUCGUGGg -3' miRNA: 3'- aaGGUGGCUAGGCcGuG-GUCAGCGUC- -5' |
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12361 | 5' | -58.6 | NC_003324.1 | + | 11753 | 0.67 | 0.476797 |
Target: 5'- gUCaauGCCGugcCCGGC-CCGGUCGCGc -3' miRNA: 3'- aAGg--UGGCua-GGCCGuGGUCAGCGUc -5' |
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12361 | 5' | -58.6 | NC_003324.1 | + | 30064 | 0.67 | 0.457053 |
Target: 5'- -gCCGCCGGUCgCGaCACCGGcCGCu- -3' miRNA: 3'- aaGGUGGCUAG-GCcGUGGUCaGCGuc -5' |
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12361 | 5' | -58.6 | NC_003324.1 | + | 14467 | 0.67 | 0.447347 |
Target: 5'- aUCgGCgGAccuugCUGGCACC-GUCGCGGa -3' miRNA: 3'- aAGgUGgCUa----GGCCGUGGuCAGCGUC- -5' |
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12361 | 5' | -58.6 | NC_003324.1 | + | 15161 | 0.68 | 0.40971 |
Target: 5'- -gCCGCCGAUCUccauCGCCGGUucCGCAGa -3' miRNA: 3'- aaGGUGGCUAGGcc--GUGGUCA--GCGUC- -5' |
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12361 | 5' | -58.6 | NC_003324.1 | + | 48729 | 0.68 | 0.40971 |
Target: 5'- -gCCaACCGcgUCGGCGCCAGcaggcUGCAGg -3' miRNA: 3'- aaGG-UGGCuaGGCCGUGGUCa----GCGUC- -5' |
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12361 | 5' | -58.6 | NC_003324.1 | + | 53289 | 0.69 | 0.364691 |
Target: 5'- -cCCGCCGGUCgCGGCuugagaaagaacuAUCGGUCGCc- -3' miRNA: 3'- aaGGUGGCUAG-GCCG-------------UGGUCAGCGuc -5' |
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12361 | 5' | -58.6 | NC_003324.1 | + | 38352 | 0.69 | 0.348817 |
Target: 5'- --gCACCGGggCGGCGCUGGUCGCc- -3' miRNA: 3'- aagGUGGCUagGCCGUGGUCAGCGuc -5' |
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12361 | 5' | -58.6 | NC_003324.1 | + | 4548 | 0.7 | 0.308683 |
Target: 5'- ---aGCCGcAUCCGGCGCCugaaucgGGUUGCAGc -3' miRNA: 3'- aaggUGGC-UAGGCCGUGG-------UCAGCGUC- -5' |
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12361 | 5' | -58.6 | NC_003324.1 | + | 3588 | 0.69 | 0.331852 |
Target: 5'- --aCGCCGAUCCaguggagcaaauuGGCAUCcguGGUCGCGGa -3' miRNA: 3'- aagGUGGCUAGG-------------CCGUGG---UCAGCGUC- -5' |
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12361 | 5' | -58.6 | NC_003324.1 | + | 18350 | 0.69 | 0.348817 |
Target: 5'- gUCgGCCGAuUCCGuCAgCGGUCGCGGc -3' miRNA: 3'- aAGgUGGCU-AGGCcGUgGUCAGCGUC- -5' |
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12361 | 5' | -58.6 | NC_003324.1 | + | 31108 | 0.67 | 0.437756 |
Target: 5'- --aCGCCGA-CC-GCGCCGGUCGCu- -3' miRNA: 3'- aagGUGGCUaGGcCGUGGUCAGCGuc -5' |
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12361 | 5' | -58.6 | NC_003324.1 | + | 33258 | 0.67 | 0.465885 |
Target: 5'- uUUCgACCGGUUcaaggagCGGUGCCAGUUGguGu -3' miRNA: 3'- -AAGgUGGCUAG-------GCCGUGGUCAGCguC- -5' |
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12361 | 5' | -58.6 | NC_003324.1 | + | 32391 | 0.67 | 0.466871 |
Target: 5'- aUCCGgaGAUgCGGCGCCg--CGCAGa -3' miRNA: 3'- aAGGUggCUAgGCCGUGGucaGCGUC- -5' |
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12361 | 5' | -58.6 | NC_003324.1 | + | 28336 | 1.05 | 0.000885 |
Target: 5'- cUUCCACCGAUCCGGCACCAGUCGCAGc -3' miRNA: 3'- -AAGGUGGCUAGGCCGUGGUCAGCGUC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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