Results 1 - 20 of 22 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
12362 | 3' | -57.1 | NC_003324.1 | + | 54424 | 0.66 | 0.645142 |
Target: 5'- cGAGAUAgugCCCgGUCaaggcGUCGAGCCa -3' miRNA: 3'- -CUCUGUa--GGGgUAGgua--CGGCUCGGg -5' |
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12362 | 3' | -57.1 | NC_003324.1 | + | 45798 | 0.66 | 0.645142 |
Target: 5'- --cACAU-CCgAUCCGUGCCGucGCCUg -3' miRNA: 3'- cucUGUAgGGgUAGGUACGGCu-CGGG- -5' |
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12362 | 3' | -57.1 | NC_003324.1 | + | 31073 | 0.66 | 0.645142 |
Target: 5'- -cGACggCCUgauUGUCCGUGCCG-GUCCu -3' miRNA: 3'- cuCUGuaGGG---GUAGGUACGGCuCGGG- -5' |
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12362 | 3' | -57.1 | NC_003324.1 | + | 28153 | 0.66 | 0.634268 |
Target: 5'- aAGGCAcgagCCCAUCCGgcaaGCCGuGGUCCg -3' miRNA: 3'- cUCUGUag--GGGUAGGUa---CGGC-UCGGG- -5' |
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12362 | 3' | -57.1 | NC_003324.1 | + | 32116 | 0.66 | 0.623392 |
Target: 5'- uGAGGCcgagugccuugGUCUCCG-CCucggGCCGGGCCg -3' miRNA: 3'- -CUCUG-----------UAGGGGUaGGua--CGGCUCGGg -5' |
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12362 | 3' | -57.1 | NC_003324.1 | + | 557 | 0.66 | 0.623392 |
Target: 5'- aGGGACGuUUCCCGauUCCAgaaaGCUGGGCgCg -3' miRNA: 3'- -CUCUGU-AGGGGU--AGGUa---CGGCUCGgG- -5' |
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12362 | 3' | -57.1 | NC_003324.1 | + | 34083 | 0.66 | 0.590845 |
Target: 5'- -cGGCuccgCCCUGUCCAUGagaCCGAGUCg -3' miRNA: 3'- cuCUGua--GGGGUAGGUAC---GGCUCGGg -5' |
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12362 | 3' | -57.1 | NC_003324.1 | + | 37040 | 0.66 | 0.590845 |
Target: 5'- uAGcCGUCuUCCAUCCA-GCCGAcGCCa -3' miRNA: 3'- cUCuGUAG-GGGUAGGUaCGGCU-CGGg -5' |
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12362 | 3' | -57.1 | NC_003324.1 | + | 21842 | 0.67 | 0.558602 |
Target: 5'- cGGACgaGUUCCUcgCCcUGCaGAGCCCg -3' miRNA: 3'- cUCUG--UAGGGGuaGGuACGgCUCGGG- -5' |
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12362 | 3' | -57.1 | NC_003324.1 | + | 55184 | 0.68 | 0.506099 |
Target: 5'- uGGGCucUCgCCCAcgCCAUGUCGAugguGCCCa -3' miRNA: 3'- cUCUGu-AG-GGGUa-GGUACGGCU----CGGG- -5' |
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12362 | 3' | -57.1 | NC_003324.1 | + | 51210 | 0.68 | 0.485684 |
Target: 5'- -cGACAUCCCCAugcuuuacUCCGgcgcgaugGuCCGcGCCCu -3' miRNA: 3'- cuCUGUAGGGGU--------AGGUa-------C-GGCuCGGG- -5' |
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12362 | 3' | -57.1 | NC_003324.1 | + | 17598 | 0.68 | 0.474623 |
Target: 5'- cAGAUAgugCCCCGgcgcgCCGUugagcacgaugccGCCGAGCUCg -3' miRNA: 3'- cUCUGUa--GGGGUa----GGUA-------------CGGCUCGGG- -5' |
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12362 | 3' | -57.1 | NC_003324.1 | + | 30372 | 0.69 | 0.465668 |
Target: 5'- -uGGCGUCgCCCucggCCAUGCC--GCCCu -3' miRNA: 3'- cuCUGUAG-GGGua--GGUACGGcuCGGG- -5' |
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12362 | 3' | -57.1 | NC_003324.1 | + | 52713 | 0.69 | 0.464679 |
Target: 5'- --cGCGUCCaucagcaCUAUCgGUGCCuGAGCCCa -3' miRNA: 3'- cucUGUAGG-------GGUAGgUACGG-CUCGGG- -5' |
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12362 | 3' | -57.1 | NC_003324.1 | + | 28282 | 0.69 | 0.44609 |
Target: 5'- -cGACuUCgCCGUCUAacgcccUGcCCGAGCCCa -3' miRNA: 3'- cuCUGuAGgGGUAGGU------AC-GGCUCGGG- -5' |
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12362 | 3' | -57.1 | NC_003324.1 | + | 20827 | 0.69 | 0.436474 |
Target: 5'- --cGCAUCgCCGUUCcgGUCGGGUCCa -3' miRNA: 3'- cucUGUAGgGGUAGGuaCGGCUCGGG- -5' |
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12362 | 3' | -57.1 | NC_003324.1 | + | 34967 | 0.69 | 0.417607 |
Target: 5'- -cGGCAUCgCCCguGUCUgugGUGuCCGAGCCUg -3' miRNA: 3'- cuCUGUAG-GGG--UAGG---UAC-GGCUCGGG- -5' |
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12362 | 3' | -57.1 | NC_003324.1 | + | 56234 | 0.71 | 0.355668 |
Target: 5'- -cGACAUCCugcauCCGUCCGUGCUucGCCa -3' miRNA: 3'- cuCUGUAGG-----GGUAGGUACGGcuCGGg -5' |
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12362 | 3' | -57.1 | NC_003324.1 | + | 22689 | 0.71 | 0.355668 |
Target: 5'- -cGACAUagagCgCAUCCAcGCCGAGCUCg -3' miRNA: 3'- cuCUGUAg---GgGUAGGUaCGGCUCGGG- -5' |
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12362 | 3' | -57.1 | NC_003324.1 | + | 57302 | 0.71 | 0.3392 |
Target: 5'- --uGCcgCCCUcgCCuccGCCGAGCCCu -3' miRNA: 3'- cucUGuaGGGGuaGGua-CGGCUCGGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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