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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
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R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 12363 | 5' | -57.7 | NC_003324.1 | + | 25973 | 0.67 | 0.465751 |
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Target: 5'- uGGUgccUUCGGCGC---CCCAGACCa-- -3' miRNA: 3'- uCCA---AAGCCGCGgacGGGUCUGGaaa -5' |
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| 12363 | 5' | -57.7 | NC_003324.1 | + | 28792 | 1.06 | 0.000845 |
|
Target: 5'- cAGGUUUCGGCGCCUGCCCAGACCUUUa -3' miRNA: 3'- -UCCAAAGCCGCGGACGGGUCUGGAAA- -5' |
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| 12363 | 5' | -57.7 | NC_003324.1 | + | 30473 | 0.67 | 0.475821 |
|
Target: 5'- cGGGUcgaaUCGGCGCC-GCCgagCAGGCCc-- -3' miRNA: 3'- -UCCAa---AGCCGCGGaCGG---GUCUGGaaa -5' |
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| 12363 | 5' | -57.7 | NC_003324.1 | + | 35685 | 0.66 | 0.570616 |
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Target: 5'- gGGGUuuucUUCGGCcugauccgggucGCUcaUGCCCGuGACCUUg -3' miRNA: 3'- -UCCA----AAGCCG------------CGG--ACGGGU-CUGGAAa -5' |
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| 12363 | 5' | -57.7 | NC_003324.1 | + | 38985 | 0.67 | 0.506656 |
|
Target: 5'- gAGGcgcUGGCGCUUGCCgAGGCUg-- -3' miRNA: 3'- -UCCaaaGCCGCGGACGGgUCUGGaaa -5' |
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| 12363 | 5' | -57.7 | NC_003324.1 | + | 40158 | 0.7 | 0.321932 |
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Target: 5'- cGGaUUUCGGCGCCgcguuccGgCCAGACCc-- -3' miRNA: 3'- uCC-AAAGCCGCGGa------CgGGUCUGGaaa -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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