Results 1 - 15 of 15 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
12365 | 5' | -55.5 | NC_003324.1 | + | 48354 | 0.66 | 0.728728 |
Target: 5'- cUGCUgCAGggGgUGGaaaauaggccCGCGugUGUGCu -3' miRNA: 3'- aGCGG-GUCuuCaACC----------GCGCugACACG- -5' |
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12365 | 5' | -55.5 | NC_003324.1 | + | 41644 | 0.69 | 0.52713 |
Target: 5'- cUCGCCCGcucucGucGgcGGCGCGACguagccgacgcgcacUGUGCc -3' miRNA: 3'- -AGCGGGU-----CuuCaaCCGCGCUG---------------ACACG- -5' |
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12365 | 5' | -55.5 | NC_003324.1 | + | 39780 | 0.68 | 0.576468 |
Target: 5'- -gGCCgGGcacaUGGCGCGAC-GUGCg -3' miRNA: 3'- agCGGgUCuucaACCGCGCUGaCACG- -5' |
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12365 | 5' | -55.5 | NC_003324.1 | + | 37135 | 0.68 | 0.565634 |
Target: 5'- uUCGgCCA-AGGUUGGCGuCGGCUGg-- -3' miRNA: 3'- -AGCgGGUcUUCAACCGC-GCUGACacg -5' |
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12365 | 5' | -55.5 | NC_003324.1 | + | 30745 | 0.67 | 0.672779 |
Target: 5'- cUGCUCGGGAGUgacggaagcacgGGCGCGGucCUGcGCc -3' miRNA: 3'- aGCGGGUCUUCAa-----------CCGCGCU--GACaCG- -5' |
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12365 | 5' | -55.5 | NC_003324.1 | + | 30611 | 0.75 | 0.247845 |
Target: 5'- cCGCCCGGAAGcaUGGUGaUGACaGUGCc -3' miRNA: 3'- aGCGGGUCUUCa-ACCGC-GCUGaCACG- -5' |
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12365 | 5' | -55.5 | NC_003324.1 | + | 29888 | 1.13 | 0.000509 |
Target: 5'- aUCGCCCAGAAGUUGGCGCGACUGUGCg -3' miRNA: 3'- -AGCGGGUCUUCAACCGCGCUGACACG- -5' |
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12365 | 5' | -55.5 | NC_003324.1 | + | 23268 | 0.66 | 0.696665 |
Target: 5'- cUUGCCCGGAAGgcucagauGCGCGccgucauaGCUGcGCa -3' miRNA: 3'- -AGCGGGUCUUCaac-----CGCGC--------UGACaCG- -5' |
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12365 | 5' | -55.5 | NC_003324.1 | + | 22865 | 0.72 | 0.385739 |
Target: 5'- gUCGCCCAgcucacGAAG-UGGCGCGACa---- -3' miRNA: 3'- -AGCGGGU------CUUCaACCGCGCUGacacg -5' |
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12365 | 5' | -55.5 | NC_003324.1 | + | 21929 | 0.67 | 0.620174 |
Target: 5'- cCGCCgAGGAGgcccggcuGCGCGaACUGaUGCa -3' miRNA: 3'- aGCGGgUCUUCaac-----CGCGC-UGAC-ACG- -5' |
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12365 | 5' | -55.5 | NC_003324.1 | + | 17163 | 0.66 | 0.685838 |
Target: 5'- -gGCCCAGcgauugacGAGgcgcUGGCGCGuCUGUcggacGCg -3' miRNA: 3'- agCGGGUC--------UUCa---ACCGCGCuGACA-----CG- -5' |
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12365 | 5' | -55.5 | NC_003324.1 | + | 14577 | 0.67 | 0.662948 |
Target: 5'- cCGCgCAGu-GUUGGCugcaccggcuuccGCGACgGUGCc -3' miRNA: 3'- aGCGgGUCuuCAACCG-------------CGCUGaCACG- -5' |
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12365 | 5' | -55.5 | NC_003324.1 | + | 11291 | 0.67 | 0.63115 |
Target: 5'- aUGCCaCGGggGUcgccucauuUGGCGUGACcGagGCg -3' miRNA: 3'- aGCGG-GUCuuCA---------ACCGCGCUGaCa-CG- -5' |
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12365 | 5' | -55.5 | NC_003324.1 | + | 9731 | 0.72 | 0.376875 |
Target: 5'- cCGCCCAGcgcGUUGGCGUugccGGCgGUGUu -3' miRNA: 3'- aGCGGGUCuu-CAACCGCG----CUGaCACG- -5' |
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12365 | 5' | -55.5 | NC_003324.1 | + | 7168 | 0.72 | 0.385739 |
Target: 5'- uUCGCgCCAacAGUUGGUGCG-CUG-GCa -3' miRNA: 3'- -AGCG-GGUcuUCAACCGCGCuGACaCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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