Results 1 - 20 of 20 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
12366 | 3' | -58.7 | NC_003324.1 | + | 13975 | 0.66 | 0.559349 |
Target: 5'- cGGGCCGGacaACGcuAUAA-CGGGCCGUc -3' miRNA: 3'- uCCUGGCCg--UGCc-UGUUaGUCCGGCA- -5' |
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12366 | 3' | -58.7 | NC_003324.1 | + | 22690 | 0.66 | 0.548828 |
Target: 5'- cGGGGCuCGGCaACGcGGCAGgcagccaaaAGGCCGc -3' miRNA: 3'- -UCCUG-GCCG-UGC-CUGUUag-------UCCGGCa -5' |
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12366 | 3' | -58.7 | NC_003324.1 | + | 42767 | 0.67 | 0.497286 |
Target: 5'- cGGAUCGGCaACGGAgAAcgugCcuuGGCCGg -3' miRNA: 3'- uCCUGGCCG-UGCCUgUUa---Gu--CCGGCa -5' |
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12366 | 3' | -58.7 | NC_003324.1 | + | 22818 | 0.67 | 0.487231 |
Target: 5'- aAGGcugcucgacGCCGGCAaaaaGGACGAU-GGGCUGa -3' miRNA: 3'- -UCC---------UGGCCGUg---CCUGUUAgUCCGGCa -5' |
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12366 | 3' | -58.7 | NC_003324.1 | + | 35785 | 0.67 | 0.477275 |
Target: 5'- aAGGucACgGGCAUGagcGACccgGAUCAGGCCGa -3' miRNA: 3'- -UCC--UGgCCGUGC---CUG---UUAGUCCGGCa -5' |
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12366 | 3' | -58.7 | NC_003324.1 | + | 603 | 0.67 | 0.467419 |
Target: 5'- uGGACCGagGCGGACAucaAGGCCu- -3' miRNA: 3'- uCCUGGCcgUGCCUGUuagUCCGGca -5' |
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12366 | 3' | -58.7 | NC_003324.1 | + | 38551 | 0.67 | 0.467419 |
Target: 5'- -uGACCGGCAgcucCGGGCGAUaGGGCaCGc -3' miRNA: 3'- ucCUGGCCGU----GCCUGUUAgUCCG-GCa -5' |
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12366 | 3' | -58.7 | NC_003324.1 | + | 9678 | 0.67 | 0.466439 |
Target: 5'- uGGGGCCGG-GCGGAUAcgCccaggucuacacgGGGUCGUg -3' miRNA: 3'- -UCCUGGCCgUGCCUGUuaG-------------UCCGGCA- -5' |
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12366 | 3' | -58.7 | NC_003324.1 | + | 18286 | 0.67 | 0.456701 |
Target: 5'- -cGGCCGacGCGCGGGCAAUCGcugccccGGCCu- -3' miRNA: 3'- ucCUGGC--CGUGCCUGUUAGU-------CCGGca -5' |
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12366 | 3' | -58.7 | NC_003324.1 | + | 28409 | 0.67 | 0.44803 |
Target: 5'- cGGGCuCGGCAUGGAUGgggauGUCucGGCUGUc -3' miRNA: 3'- uCCUG-GCCGUGCCUGU-----UAGu-CCGGCA- -5' |
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12366 | 3' | -58.7 | NC_003324.1 | + | 24385 | 0.68 | 0.429095 |
Target: 5'- cGGGCUGGC-CGaGAUggUCGGGCa-- -3' miRNA: 3'- uCCUGGCCGuGC-CUGuuAGUCCGgca -5' |
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12366 | 3' | -58.7 | NC_003324.1 | + | 24586 | 0.68 | 0.429095 |
Target: 5'- gGGcGACCaGCGCaGGAUcAUCGGGgCCGUa -3' miRNA: 3'- -UC-CUGGcCGUG-CCUGuUAGUCC-GGCA- -5' |
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12366 | 3' | -58.7 | NC_003324.1 | + | 4866 | 0.68 | 0.392686 |
Target: 5'- -cGGCCGGUcgagcccuguucAUGGGCcGUCAGGCUGg -3' miRNA: 3'- ucCUGGCCG------------UGCCUGuUAGUCCGGCa -5' |
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12366 | 3' | -58.7 | NC_003324.1 | + | 11829 | 0.69 | 0.358345 |
Target: 5'- cGGGCCgGGCACGG-CAuugaccacGUUGGGCCa- -3' miRNA: 3'- uCCUGG-CCGUGCCuGU--------UAGUCCGGca -5' |
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12366 | 3' | -58.7 | NC_003324.1 | + | 14519 | 0.7 | 0.34198 |
Target: 5'- uGGacGCCGGCAUGGGCc--CAGaGCCGg -3' miRNA: 3'- uCC--UGGCCGUGCCUGuuaGUC-CGGCa -5' |
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12366 | 3' | -58.7 | NC_003324.1 | + | 45688 | 0.71 | 0.282003 |
Target: 5'- aGGGGCuCGGCGaggcgugagcaGGGCAAUCAGGCg-- -3' miRNA: 3'- -UCCUG-GCCGUg----------CCUGUUAGUCCGgca -5' |
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12366 | 3' | -58.7 | NC_003324.1 | + | 12522 | 0.71 | 0.279925 |
Target: 5'- cGGGCUGGUggcaguucgaggcgACGGuuCGAUCAGGCUGg -3' miRNA: 3'- uCCUGGCCG--------------UGCCu-GUUAGUCCGGCa -5' |
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12366 | 3' | -58.7 | NC_003324.1 | + | 35146 | 0.74 | 0.186963 |
Target: 5'- cGGGcgacaaugcagaaACCGGCAgCGacGACGAUCAGGCCGc -3' miRNA: 3'- -UCC-------------UGGCCGU-GC--CUGUUAGUCCGGCa -5' |
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12366 | 3' | -58.7 | NC_003324.1 | + | 11216 | 0.75 | 0.155652 |
Target: 5'- -aGACCGGCgaaGCGGACcaaGGUCGGGCCa- -3' miRNA: 3'- ucCUGGCCG---UGCCUG---UUAGUCCGGca -5' |
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12366 | 3' | -58.7 | NC_003324.1 | + | 31158 | 1.06 | 0.00076 |
Target: 5'- aAGGACCGGCACGGACAAUCAGGCCGUc -3' miRNA: 3'- -UCCUGGCCGUGCCUGUUAGUCCGGCA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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