Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 12367 | 3' | -56.5 | NC_003324.1 | + | 55047 | 0.66 | 0.641562 |
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Target: 5'- -cGGACGGCuGGGCUgccGCGACgGcgCAGa -3' miRNA: 3'- cuCCUGCCG-CCUGA---CGCUGgUaaGUU- -5' |
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| 12367 | 3' | -56.5 | NC_003324.1 | + | 53356 | 0.66 | 0.608505 |
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Target: 5'- --cGAcCGGCGGGC-GCGAUCAUUCc- -3' miRNA: 3'- cucCU-GCCGCCUGaCGCUGGUAAGuu -5' |
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| 12367 | 3' | -56.5 | NC_003324.1 | + | 53192 | 0.66 | 0.597514 |
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Target: 5'- -uGGugGGUGGuaGCUgGCGGCCGgcUUCAc -3' miRNA: 3'- cuCCugCCGCC--UGA-CGCUGGU--AAGUu -5' |
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| 12367 | 3' | -56.5 | NC_003324.1 | + | 12994 | 0.67 | 0.586553 |
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Target: 5'- aGAGaGAUGGCGGAaccaCUGuCGACCAa---- -3' miRNA: 3'- -CUC-CUGCCGCCU----GAC-GCUGGUaaguu -5' |
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| 12367 | 3' | -56.5 | NC_003324.1 | + | 20801 | 0.67 | 0.569099 |
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Target: 5'- cGAGG-CGGCGGACcGUGuuggugacgcgcaucGCCGUUCc- -3' miRNA: 3'- -CUCCuGCCGCCUGaCGC---------------UGGUAAGuu -5' |
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| 12367 | 3' | -56.5 | NC_003324.1 | + | 24004 | 0.69 | 0.449238 |
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Target: 5'- uAGGGCGGCGGuagggcuGCUGCGugCGcUUgGAg -3' miRNA: 3'- cUCCUGCCGCC-------UGACGCugGU-AAgUU- -5' |
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| 12367 | 3' | -56.5 | NC_003324.1 | + | 52302 | 0.69 | 0.421204 |
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Target: 5'- cGGGGCGGcCGGcguCUGCGACCccagCAAg -3' miRNA: 3'- cUCCUGCC-GCCu--GACGCUGGuaa-GUU- -5' |
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| 12367 | 3' | -56.5 | NC_003324.1 | + | 16025 | 0.72 | 0.280021 |
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Target: 5'- -uGGACGGCGGACUGgcuggugaugaGGCCAagUUCGAc -3' miRNA: 3'- cuCCUGCCGCCUGACg----------CUGGU--AAGUU- -5' |
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| 12367 | 3' | -56.5 | NC_003324.1 | + | 31592 | 1.06 | 0.001139 |
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Target: 5'- cGAGGACGGCGGACUGCGACCAUUCAAu -3' miRNA: 3'- -CUCCUGCCGCCUGACGCUGGUAAGUU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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