miRNA: 3'- gCG-GCU--------CCGACGGc---CGCUUCCGCuGC- -5'
miRNA: 3'- gCGGCuCCGACGGcCGCUuCCGCuGc -5'
miRNA: 3'- gCGGCu-CCGA-----CGGCCGCUUc--CGCUGC- -5'
miRNA: 3'- gCGGCUCCga-CgGCCGCUUCCGCuGC- -5'
miRNA: 3'- gCGGC--UCCGA--CGg--CCGCUUCCGCUGc -5'
Results 61 - 65 of 65 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
|
R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 12369 | 5' | -62.4 | NC_003324.1 | + | 54215 | 0.66 | 0.381296 |
|
Target: 5'- aGCgCGAcauagagcGGCUGCUcaaaGCGAAGGCG-CGg -3' miRNA: 3'- gCG-GCU--------CCGACGGc---CGCUUCCGCuGC- -5' |
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| 12369 | 5' | -62.4 | NC_003324.1 | + | 55052 | 0.67 | 0.333077 |
|
Target: 5'- gGCUG-GGCUGCC-GCGAcGGCG-Ca -3' miRNA: 3'- gCGGCuCCGACGGcCGCUuCCGCuGc -5' |
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| 12369 | 5' | -62.4 | NC_003324.1 | + | 56060 | 0.68 | 0.275982 |
|
Target: 5'- gGCCGucGGCUuccagGCUGGCGAAcccGCGAUGg -3' miRNA: 3'- gCGGCu-CCGA-----CGGCCGCUUc--CGCUGC- -5' |
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| 12369 | 5' | -62.4 | NC_003324.1 | + | 57038 | 0.72 | 0.154925 |
|
Target: 5'- gGCUGGGGagcGgCGGCGAGGGCG-CGg -3' miRNA: 3'- gCGGCUCCga-CgGCCGCUUCCGCuGC- -5' |
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| 12369 | 5' | -62.4 | NC_003324.1 | + | 57390 | 0.72 | 0.147053 |
|
Target: 5'- uGCUGgaGGGCUcgGCggaGGCGAGGGCGGCa -3' miRNA: 3'- gCGGC--UCCGA--CGg--CCGCUUCCGCUGc -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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