miRNA: 3'- gcGGCUCCga---CGGCCGCuUCCGCUGC- -5'
miRNA: 3'- -GCGGCUCCGa----CG--GCCGCuuCCGCUGC- -5'
miRNA: 3'- -GCG---GCUCCGACG-----GCCGCUu-CCGCUGC- -5'
miRNA: 3'- -GCGGCUCCgac---GGCCGCUUCCGCUGc -5'
miRNA: 3'- -GCGGCUCCGACGGCCGCUUCCGCUGC- -5'
Results 61 - 65 of 65 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 12369 | 5' | -62.4 | NC_003324.1 | + | 21388 | 0.73 | 0.125581 |
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Target: 5'- -aUCGAGGauaucGCCGGCG-AGGCGGCGa -3' miRNA: 3'- gcGGCUCCga---CGGCCGCuUCCGCUGC- -5' |
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| 12369 | 5' | -62.4 | NC_003324.1 | + | 35209 | 0.73 | 0.125581 |
|
Target: 5'- gCGCUGAGGUcaacgGCgaCGGCGcuGGCGACGa -3' miRNA: 3'- -GCGGCUCCGa----CG--GCCGCuuCCGCUGC- -5' |
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| 12369 | 5' | -62.4 | NC_003324.1 | + | 13179 | 0.75 | 0.098771 |
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Target: 5'- cCGUcagCGAGGCUGCgucggCGGCGGucGGCGGCGg -3' miRNA: 3'- -GCG---GCUCCGACG-----GCCGCUu-CCGCUGC- -5' |
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| 12369 | 5' | -62.4 | NC_003324.1 | + | 25651 | 0.79 | 0.051329 |
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Target: 5'- cCGCCGAGGacaaucUCGGCGAAGGCGGCc -3' miRNA: 3'- -GCGGCUCCgac---GGCCGCUUCCGCUGc -5' |
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| 12369 | 5' | -62.4 | NC_003324.1 | + | 32555 | 1.08 | 0.000279 |
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Target: 5'- uCGCCGAGGCUGCCGGCGAAGGCGACGa -3' miRNA: 3'- -GCGGCUCCGACGGCCGCUUCCGCUGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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