Results 21 - 23 of 23 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
12370 | 3' | -55.4 | NC_003324.1 | + | 43919 | 0.66 | 0.694142 |
Target: 5'- ----uGGCgCCGgCACcGAGCCCGCa- -3' miRNA: 3'- guauuCCG-GGCaGUGuUUCGGGCGgu -5' |
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12370 | 3' | -55.4 | NC_003324.1 | + | 53886 | 0.66 | 0.67221 |
Target: 5'- aAUGGGGCCUGgugCGC----CCCGCCGc -3' miRNA: 3'- gUAUUCCGGGCa--GUGuuucGGGCGGU- -5' |
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12370 | 3' | -55.4 | NC_003324.1 | + | 55131 | 0.66 | 0.705023 |
Target: 5'- -----uGCgCCGUCGCGGcAGCCCaGCCGu -3' miRNA: 3'- guauucCG-GGCAGUGUU-UCGGG-CGGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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