Results 1 - 20 of 23 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
12370 | 3' | -55.4 | NC_003324.1 | + | 616 | 0.68 | 0.561903 |
Target: 5'- aCAUcAAGGCCUGgcaGCuGGGCuuGCCAu -3' miRNA: 3'- -GUA-UUCCGGGCag-UGuUUCGggCGGU- -5' |
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12370 | 3' | -55.4 | NC_003324.1 | + | 4587 | 0.66 | 0.715832 |
Target: 5'- --cGAGGCCUuaguaGUCGCGucGCC-GCCGu -3' miRNA: 3'- guaUUCCGGG-----CAGUGUuuCGGgCGGU- -5' |
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12370 | 3' | -55.4 | NC_003324.1 | + | 8303 | 0.68 | 0.561903 |
Target: 5'- uCGUGAcGCCCGUCACAGagauGGUCgaagUGCCAg -3' miRNA: 3'- -GUAUUcCGGGCAGUGUU----UCGG----GCGGU- -5' |
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12370 | 3' | -55.4 | NC_003324.1 | + | 10260 | 0.66 | 0.705023 |
Target: 5'- --gAAGGUCCGg-ACAAAGCUguaGCCAu -3' miRNA: 3'- guaUUCCGGGCagUGUUUCGGg--CGGU- -5' |
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12370 | 3' | -55.4 | NC_003324.1 | + | 14058 | 0.68 | 0.583787 |
Target: 5'- ----cGGCCCGUUAUAGcguuguccGGCCCGUUu -3' miRNA: 3'- guauuCCGGGCAGUGUU--------UCGGGCGGu -5' |
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12370 | 3' | -55.4 | NC_003324.1 | + | 14482 | 0.67 | 0.650125 |
Target: 5'- ----uGGCaCCGUCGCGgAAGCCggugcaGCCAa -3' miRNA: 3'- guauuCCG-GGCAGUGU-UUCGGg-----CGGU- -5' |
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12370 | 3' | -55.4 | NC_003324.1 | + | 16308 | 0.68 | 0.561903 |
Target: 5'- ----uGGCCgCGUCgGCAcGGCCUGCCc -3' miRNA: 3'- guauuCCGG-GCAG-UGUuUCGGGCGGu -5' |
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12370 | 3' | -55.4 | NC_003324.1 | + | 18099 | 0.66 | 0.694142 |
Target: 5'- ----uGGCCCGUgGC---GCCgGCCAa -3' miRNA: 3'- guauuCCGGGCAgUGuuuCGGgCGGU- -5' |
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12370 | 3' | -55.4 | NC_003324.1 | + | 18480 | 0.68 | 0.594794 |
Target: 5'- uCGUGuGGUUCcUCGCAGGGUCCGUCAg -3' miRNA: 3'- -GUAUuCCGGGcAGUGUUUCGGGCGGU- -5' |
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12370 | 3' | -55.4 | NC_003324.1 | + | 21441 | 0.66 | 0.705023 |
Target: 5'- -cUAAGGCCCGguUCAUccagcAGGUCCGCa- -3' miRNA: 3'- guAUUCCGGGC--AGUGu----UUCGGGCGgu -5' |
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12370 | 3' | -55.4 | NC_003324.1 | + | 23426 | 0.69 | 0.529525 |
Target: 5'- aAUGAcGCCCGcCGCGacGAGCUCGUCAg -3' miRNA: 3'- gUAUUcCGGGCaGUGU--UUCGGGCGGU- -5' |
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12370 | 3' | -55.4 | NC_003324.1 | + | 25175 | 0.67 | 0.616896 |
Target: 5'- ---cGGGCgCCGUCGCGcAGCUCaCCAg -3' miRNA: 3'- guauUCCG-GGCAGUGUuUCGGGcGGU- -5' |
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12370 | 3' | -55.4 | NC_003324.1 | + | 31152 | 0.67 | 0.650125 |
Target: 5'- ---cAGGCCUGccUCGCGGAGCUgcccggccugCGCCAu -3' miRNA: 3'- guauUCCGGGC--AGUGUUUCGG----------GCGGU- -5' |
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12370 | 3' | -55.4 | NC_003324.1 | + | 32731 | 0.67 | 0.627972 |
Target: 5'- ---uGGGCaaaa-ACAAAGCCCGCCGa -3' miRNA: 3'- guauUCCGggcagUGUUUCGGGCGGU- -5' |
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12370 | 3' | -55.4 | NC_003324.1 | + | 32845 | 1.08 | 0.001093 |
Target: 5'- gCAUAAGGCCCGUCACAAAGCCCGCCAc -3' miRNA: 3'- -GUAUUCCGGGCAGUGUUUCGGGCGGU- -5' |
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12370 | 3' | -55.4 | NC_003324.1 | + | 32921 | 0.67 | 0.643482 |
Target: 5'- --aAAGGCgCGUCagugGCGAugaucgaggcggcuGCCCGCCAu -3' miRNA: 3'- guaUUCCGgGCAG----UGUUu-------------CGGGCGGU- -5' |
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12370 | 3' | -55.4 | NC_003324.1 | + | 36441 | 0.7 | 0.447092 |
Target: 5'- cCGUAGGGCaugCCGUCGC---GCCCGCg- -3' miRNA: 3'- -GUAUUCCG---GGCAGUGuuuCGGGCGgu -5' |
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12370 | 3' | -55.4 | NC_003324.1 | + | 43919 | 0.66 | 0.694142 |
Target: 5'- ----uGGCgCCGgCACcGAGCCCGCa- -3' miRNA: 3'- guauuCCG-GGCaGUGuUUCGGGCGgu -5' |
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12370 | 3' | -55.4 | NC_003324.1 | + | 49321 | 0.7 | 0.447092 |
Target: 5'- ---cGGGCCCGUCGgGcuGCCCGaUCAu -3' miRNA: 3'- guauUCCGGGCAGUgUuuCGGGC-GGU- -5' |
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12370 | 3' | -55.4 | NC_003324.1 | + | 52108 | 0.73 | 0.291481 |
Target: 5'- --aGAGGCCuCGcgaacauaCugAGAGCCCGCCAa -3' miRNA: 3'- guaUUCCGG-GCa-------GugUUUCGGGCGGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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