miRNA: 3'- -GUAUUCCG---GGCAGUGuuuCGGGCGgu -5'
miRNA: 3'- guaUUCCGG-GCa-------GugUUUCGGGCGGU- -5'
miRNA: 3'- -GUAUUCCGGGCAGUGUUUCGGGCGGU- -5'
Results 21 - 23 of 23 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 12370 | 3' | -55.4 | NC_003324.1 | + | 36441 | 0.7 | 0.447092 |
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Target: 5'- cCGUAGGGCaugCCGUCGC---GCCCGCg- -3' miRNA: 3'- -GUAUUCCG---GGCAGUGuuuCGGGCGgu -5' |
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| 12370 | 3' | -55.4 | NC_003324.1 | + | 52108 | 0.73 | 0.291481 |
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Target: 5'- --aGAGGCCuCGcgaacauaCugAGAGCCCGCCAa -3' miRNA: 3'- guaUUCCGG-GCa-------GugUUUCGGGCGGU- -5' |
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| 12370 | 3' | -55.4 | NC_003324.1 | + | 32845 | 1.08 | 0.001093 |
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Target: 5'- gCAUAAGGCCCGUCACAAAGCCCGCCAc -3' miRNA: 3'- -GUAUUCCGGGCAGUGUUUCGGGCGGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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