Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
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Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 12371 | 5' | -52.7 | NC_003324.1 | + | 36293 | 0.68 | 0.729817 |
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Target: 5'- --aUCGggGCGGGCGauUUCuUCCGCCAGc -3' miRNA: 3'- gcaAGUagCGUUCGC--AAG-AGGCGGUC- -5' |
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| 12371 | 5' | -52.7 | NC_003324.1 | + | 28282 | 0.68 | 0.751053 |
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Target: 5'- ---aCAUCGCuugcGCGUUCggCGCCGGa -3' miRNA: 3'- gcaaGUAGCGuu--CGCAAGagGCGGUC- -5' |
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| 12371 | 5' | -52.7 | NC_003324.1 | + | 9024 | 0.68 | 0.771791 |
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Target: 5'- ---cCAcCGCAaaGGCGUUUgCCGCCGGg -3' miRNA: 3'- gcaaGUaGCGU--UCGCAAGaGGCGGUC- -5' |
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| 12371 | 5' | -52.7 | NC_003324.1 | + | 4912 | 0.66 | 0.839113 |
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Target: 5'- -uUUCGgcucCGCAGGCGgcUUCCGCUAc -3' miRNA: 3'- gcAAGUa---GCGUUCGCaaGAGGCGGUc -5' |
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| 12371 | 5' | -52.7 | NC_003324.1 | + | 47515 | 0.67 | 0.820844 |
|
Target: 5'- gGUUCAgagcguagUgGCAGGCGUcgaagcugugCUCCGCCuGg -3' miRNA: 3'- gCAAGU--------AgCGUUCGCAa---------GAGGCGGuC- -5' |
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| 12371 | 5' | -52.7 | NC_003324.1 | + | 2600 | 0.69 | 0.70818 |
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Target: 5'- uGUUCGUCGguGGCGUcacggcuuacuaUCUgUCGCCGc -3' miRNA: 3'- gCAAGUAGCguUCGCA------------AGA-GGCGGUc -5' |
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| 12371 | 5' | -52.7 | NC_003324.1 | + | 11687 | 0.72 | 0.520667 |
|
Target: 5'- aCGUUCAUCGCugcCGUcgcaccagUCUCCGgCAGg -3' miRNA: 3'- -GCAAGUAGCGuucGCA--------AGAGGCgGUC- -5' |
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| 12371 | 5' | -52.7 | NC_003324.1 | + | 49295 | 0.72 | 0.510017 |
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Target: 5'- ---gCGUCGCAGGCGUUCggaUCGUCGGc -3' miRNA: 3'- gcaaGUAGCGUUCGCAAGa--GGCGGUC- -5' |
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| 12371 | 5' | -52.7 | NC_003324.1 | + | 33479 | 0.97 | 0.013727 |
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Target: 5'- gCGUUCAUCGCAAGC-UUCUCCGCCAGu -3' miRNA: 3'- -GCAAGUAGCGUUCGcAAGAGGCGGUC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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