miRNA: 3'- cugaUGUaGCUACACCUGGAGAUGcu -5'
miRNA: 3'- -CUgaUGUAGCUAca--C-CUGGAGAUGCU- -5'
miRNA: 3'- cugAUGUaGCU---ACACCUGGAGa---UGCU- -5'
miRNA: 3'- cUGaUGu-AGCUAca-CCUGGAGAUGCU- -5'
miRNA: 3'- -CUGAUGUAGCUACACCUGGAGAUGCU- -5'
Results 1 - 5 of 5 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
|
Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 12373 | 3' | -49.9 | NC_003324.1 | + | 10377 | 0.73 | 0.622099 |
|
Target: 5'- ---gACA-CGAUGUGGAUCUCUGCa- -3' miRNA: 3'- cugaUGUaGCUACACCUGGAGAUGcu -5' |
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| 12373 | 3' | -49.9 | NC_003324.1 | + | 37579 | 0.71 | 0.734462 |
|
Target: 5'- uGAggGCGUCGAUcaugGuGACCUCUGCGAc -3' miRNA: 3'- -CUgaUGUAGCUAca--C-CUGGAGAUGCU- -5' |
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| 12373 | 3' | -49.9 | NC_003324.1 | + | 5868 | 0.66 | 0.952992 |
|
Target: 5'- --gUGCA-CGAgauUGUGGACCUCagaaACGAa -3' miRNA: 3'- cugAUGUaGCU---ACACCUGGAGa---UGCU- -5' |
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| 12373 | 3' | -49.9 | NC_003324.1 | + | 41524 | 0.69 | 0.853713 |
|
Target: 5'- uGCcGCcuUCGGUuugGGACCUCUACGAg -3' miRNA: 3'- cUGaUGu-AGCUAca-CCUGGAGAUGCU- -5' |
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| 12373 | 3' | -49.9 | NC_003324.1 | + | 33936 | 1.09 | 0.003703 |
|
Target: 5'- gGACUACAUCGAUGUGGACCUCUACGAu -3' miRNA: 3'- -CUGAUGUAGCUACACCUGGAGAUGCU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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