Results 1 - 16 of 16 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
12373 | 5' | -50.6 | NC_003324.1 | + | 33898 | 1.11 | 0.002166 |
Target: 5'- cGGCCGUGCAAGCCGCAAUCGAUAUAGc -3' miRNA: 3'- -CCGGCACGUUCGGCGUUAGCUAUAUC- -5' |
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12373 | 5' | -50.6 | NC_003324.1 | + | 30075 | 0.73 | 0.603407 |
Target: 5'- cGCCagaGUGCAccGGCCGgGAUCGAUGcGGa -3' miRNA: 3'- cCGG---CACGU--UCGGCgUUAGCUAUaUC- -5' |
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12373 | 5' | -50.6 | NC_003324.1 | + | 20387 | 0.72 | 0.656779 |
Target: 5'- cGGCCGUGCcguauucgacaacgGAGCCGuCAucGUCGAUc--- -3' miRNA: 3'- -CCGGCACG--------------UUCGGC-GU--UAGCUAuauc -5' |
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12373 | 5' | -50.6 | NC_003324.1 | + | 51975 | 0.7 | 0.738136 |
Target: 5'- aGGCCc-GCAAGCCGCAuUCcugcAUAUGGu -3' miRNA: 3'- -CCGGcaCGUUCGGCGUuAGc---UAUAUC- -5' |
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12373 | 5' | -50.6 | NC_003324.1 | + | 12187 | 0.7 | 0.748917 |
Target: 5'- aGGCCGcGCGcuCCGCGAUCaGGUAcAGa -3' miRNA: 3'- -CCGGCaCGUucGGCGUUAG-CUAUaUC- -5' |
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12373 | 5' | -50.6 | NC_003324.1 | + | 14646 | 0.69 | 0.823946 |
Target: 5'- cGCCG-GCAGGCCGCGacccauguugaccacGUCGGc---- -3' miRNA: 3'- cCGGCaCGUUCGGCGU---------------UAGCUauauc -5' |
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12373 | 5' | -50.6 | NC_003324.1 | + | 31956 | 0.68 | 0.856485 |
Target: 5'- aGGCCGguuUGCAguuccGGCUucauGCucUCGAUGUAGg -3' miRNA: 3'- -CCGGC---ACGU-----UCGG----CGuuAGCUAUAUC- -5' |
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12373 | 5' | -50.6 | NC_003324.1 | + | 48491 | 0.68 | 0.829576 |
Target: 5'- aGGCgGUGaagcguuccaAGGCCGCAGcCGAgcagGUAGg -3' miRNA: 3'- -CCGgCACg---------UUCGGCGUUaGCUa---UAUC- -5' |
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12373 | 5' | -50.6 | NC_003324.1 | + | 5252 | 0.68 | 0.83878 |
Target: 5'- uGGCCGccGCAccAGCgGCAggCGAUAUc- -3' miRNA: 3'- -CCGGCa-CGU--UCGgCGUuaGCUAUAuc -5' |
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12373 | 5' | -50.6 | NC_003324.1 | + | 39460 | 0.67 | 0.881145 |
Target: 5'- aGCCGcagGCAAGCUGCuucgccgGAUGUGGc -3' miRNA: 3'- cCGGCa--CGUUCGGCGuuag---CUAUAUC- -5' |
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12373 | 5' | -50.6 | NC_003324.1 | + | 8915 | 0.67 | 0.896233 |
Target: 5'- cGGCCGUGUugauagauGCUGCGagcGUCGAg---- -3' miRNA: 3'- -CCGGCACGuu------CGGCGU---UAGCUauauc -5' |
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12373 | 5' | -50.6 | NC_003324.1 | + | 4449 | 0.67 | 0.896233 |
Target: 5'- gGGCCGgGCAuGCUGCAAaccCGAUucAGg -3' miRNA: 3'- -CCGGCaCGUuCGGCGUUa--GCUAuaUC- -5' |
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12373 | 5' | -50.6 | NC_003324.1 | + | 16319 | 0.66 | 0.922975 |
Target: 5'- gGGCCcUGCGcuGGCCGC-GUCGGc---- -3' miRNA: 3'- -CCGGcACGU--UCGGCGuUAGCUauauc -5' |
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12373 | 5' | -50.6 | NC_003324.1 | + | 31546 | 0.66 | 0.934587 |
Target: 5'- uGGCCGccacGCGcGcCCGCGGucUCGAUAUGc -3' miRNA: 3'- -CCGGCa---CGUuC-GGCGUU--AGCUAUAUc -5' |
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12373 | 5' | -50.6 | NC_003324.1 | + | 50387 | 0.66 | 0.928929 |
Target: 5'- cGCCGuUGUcccGGGCCGCGAcauguUCGGUGa-- -3' miRNA: 3'- cCGGC-ACG---UUCGGCGUU-----AGCUAUauc -5' |
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12373 | 5' | -50.6 | NC_003324.1 | + | 35458 | 0.66 | 0.910186 |
Target: 5'- cGGCCGaGCAGGCUGCAGc-------- -3' miRNA: 3'- -CCGGCaCGUUCGGCGUUagcuauauc -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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