Results 21 - 36 of 36 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
12374 | 5' | -55.8 | NC_003324.1 | + | 13926 | 0.67 | 0.652215 |
Target: 5'- -aAUGC-UGCgGAgcgaGUGCCGUUGGCUc -3' miRNA: 3'- gcUGCGaACGgUUa---CACGGCAGCCGG- -5' |
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12374 | 5' | -55.8 | NC_003324.1 | + | 31225 | 0.67 | 0.652215 |
Target: 5'- aCGAUGCccccGCCAGcgccaGcGCCGcCGGCCg -3' miRNA: 3'- -GCUGCGaa--CGGUUa----CaCGGCaGCCGG- -5' |
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12374 | 5' | -55.8 | NC_003324.1 | + | 49469 | 0.67 | 0.656564 |
Target: 5'- cCGACGaggacuaUGCCAagGUGCCGguccgcaacgagggCGGCa -3' miRNA: 3'- -GCUGCga-----ACGGUuaCACGGCa-------------GCCGg -5' |
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12374 | 5' | -55.8 | NC_003324.1 | + | 18426 | 0.67 | 0.673917 |
Target: 5'- gCGACGCUggucauggucUGCgCGAccuUGCCGcCGGUCg -3' miRNA: 3'- -GCUGCGA----------ACG-GUUac-ACGGCaGCCGG- -5' |
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12374 | 5' | -55.8 | NC_003324.1 | + | 57047 | 0.67 | 0.673917 |
Target: 5'- aCGACGCUcGCaa----GCCGUCuuGGCCu -3' miRNA: 3'- -GCUGCGAaCGguuacaCGGCAG--CCGG- -5' |
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12374 | 5' | -55.8 | NC_003324.1 | + | 39252 | 0.67 | 0.66308 |
Target: 5'- cCGugGCUgcGCCAGaagGUGCCGaUCGucaCCg -3' miRNA: 3'- -GCugCGAa-CGGUUa--CACGGC-AGCc--GG- -5' |
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12374 | 5' | -55.8 | NC_003324.1 | + | 54230 | 0.66 | 0.695464 |
Target: 5'- uGACGCg-GCCAGcuc-CCGUCGaGCCa -3' miRNA: 3'- gCUGCGaaCGGUUacacGGCAGC-CGG- -5' |
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12374 | 5' | -55.8 | NC_003324.1 | + | 14544 | 0.66 | 0.716771 |
Target: 5'- uGGCGCUcaagGCCGAcguggucaacaUGgGUCG-CGGCCu -3' miRNA: 3'- gCUGCGAa---CGGUU-----------ACaCGGCaGCCGG- -5' |
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12374 | 5' | -55.8 | NC_003324.1 | + | 27882 | 0.66 | 0.716771 |
Target: 5'- uGGCGUagccgGCCAuuaccuUGUGCuCGUcgaagCGGCCa -3' miRNA: 3'- gCUGCGaa---CGGUu-----ACACG-GCA-----GCCGG- -5' |
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12374 | 5' | -55.8 | NC_003324.1 | + | 38360 | 0.66 | 0.716771 |
Target: 5'- gCGGCGCUggucGCCGucGUGCgGgaggaaguggUGGCCg -3' miRNA: 3'- -GCUGCGAa---CGGUuaCACGgCa---------GCCGG- -5' |
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12374 | 5' | -55.8 | NC_003324.1 | + | 57283 | 0.66 | 0.727307 |
Target: 5'- aGAUGCUcaacGCCGGUcGUGCCGcCcucGCCu -3' miRNA: 3'- gCUGCGAa---CGGUUA-CACGGCaGc--CGG- -5' |
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12374 | 5' | -55.8 | NC_003324.1 | + | 18234 | 0.66 | 0.727307 |
Target: 5'- aCGAC-CUUGCCGGUGUcGgCGaaacCGGCg -3' miRNA: 3'- -GCUGcGAACGGUUACA-CgGCa---GCCGg -5' |
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12374 | 5' | -55.8 | NC_003324.1 | + | 50087 | 0.66 | 0.727307 |
Target: 5'- -aGCGCUUGCCGAUcuUGUgGgCGGCg -3' miRNA: 3'- gcUGCGAACGGUUAc-ACGgCaGCCGg -5' |
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12374 | 5' | -55.8 | NC_003324.1 | + | 22494 | 0.66 | 0.716771 |
Target: 5'- uGACGCUUGgCGAagGUGU--UCGGCg -3' miRNA: 3'- gCUGCGAACgGUUa-CACGgcAGCCGg -5' |
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12374 | 5' | -55.8 | NC_003324.1 | + | 5338 | 0.66 | 0.695464 |
Target: 5'- gGAuauCGCcUGCCGcugGUGCgG-CGGCCa -3' miRNA: 3'- gCU---GCGaACGGUua-CACGgCaGCCGG- -5' |
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12374 | 5' | -55.8 | NC_003324.1 | + | 47661 | 0.66 | 0.727307 |
Target: 5'- uGugGCacGCCGGUGcGCuggacaguucCGUCGGCg -3' miRNA: 3'- gCugCGaaCGGUUACaCG----------GCAGCCGg -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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