Results 21 - 36 of 36 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
12374 | 5' | -55.8 | NC_003324.1 | + | 6385 | 0.68 | 0.597813 |
Target: 5'- --gUGCUcGCCAA---GCCGUCGGCUu -3' miRNA: 3'- gcuGCGAaCGGUUacaCGGCAGCCGG- -5' |
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12374 | 5' | -55.8 | NC_003324.1 | + | 15623 | 0.68 | 0.597813 |
Target: 5'- uCGGCGCUUuCC-AUGUcGCCGUUGGa- -3' miRNA: 3'- -GCUGCGAAcGGuUACA-CGGCAGCCgg -5' |
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12374 | 5' | -55.8 | NC_003324.1 | + | 54341 | 0.68 | 0.597813 |
Target: 5'- uCGGCGCUgaUGCCGGUccauggcaaccgGUGCCG-C-GCCu -3' miRNA: 3'- -GCUGCGA--ACGGUUA------------CACGGCaGcCGG- -5' |
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12374 | 5' | -55.8 | NC_003324.1 | + | 16225 | 0.68 | 0.586982 |
Target: 5'- aCGGgGCagGCC---GUGCCGacgCGGCCa -3' miRNA: 3'- -GCUgCGaaCGGuuaCACGGCa--GCCGG- -5' |
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12374 | 5' | -55.8 | NC_003324.1 | + | 47877 | 0.68 | 0.56544 |
Target: 5'- cCGGCGUcaggaccuUUGCCGaaccGUGUgcGCCGaUGGCCg -3' miRNA: 3'- -GCUGCG--------AACGGU----UACA--CGGCaGCCGG- -5' |
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12374 | 5' | -55.8 | NC_003324.1 | + | 47566 | 0.68 | 0.56544 |
Target: 5'- uGACGaggGCCGAUcgacccucGCCGUCGaGCCg -3' miRNA: 3'- gCUGCgaaCGGUUAca------CGGCAGC-CGG- -5' |
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12374 | 5' | -55.8 | NC_003324.1 | + | 8088 | 0.68 | 0.554745 |
Target: 5'- uGACcaugGC-UGCCAcgGUcGCCGUUGcGCCu -3' miRNA: 3'- gCUG----CGaACGGUuaCA-CGGCAGC-CGG- -5' |
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12374 | 5' | -55.8 | NC_003324.1 | + | 53737 | 0.69 | 0.502306 |
Target: 5'- uCGGCGCU--CCAagGUGCCGcaCGGCg -3' miRNA: 3'- -GCUGCGAacGGUuaCACGGCa-GCCGg -5' |
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12374 | 5' | -55.8 | NC_003324.1 | + | 27684 | 0.7 | 0.481933 |
Target: 5'- uGGCGCcgagcuucuccUUGgCGAUGUGCgCcUCGGCCu -3' miRNA: 3'- gCUGCG-----------AACgGUUACACG-GcAGCCGG- -5' |
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12374 | 5' | -55.8 | NC_003324.1 | + | 46405 | 0.7 | 0.442448 |
Target: 5'- aGGCGCUUGCgguaguccugCAGUGU-UCGcCGGCCg -3' miRNA: 3'- gCUGCGAACG----------GUUACAcGGCaGCCGG- -5' |
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12374 | 5' | -55.8 | NC_003324.1 | + | 6314 | 0.71 | 0.404855 |
Target: 5'- uGGCGCUUGCUgcgcUGcUGCUGcgccUCGGCCu -3' miRNA: 3'- gCUGCGAACGGuu--AC-ACGGC----AGCCGG- -5' |
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12374 | 5' | -55.8 | NC_003324.1 | + | 29065 | 0.72 | 0.378017 |
Target: 5'- uCGACGC-UGCCGGUGaagaUGCauUCGGUCa -3' miRNA: 3'- -GCUGCGaACGGUUAC----ACGgcAGCCGG- -5' |
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12374 | 5' | -55.8 | NC_003324.1 | + | 38978 | 0.72 | 0.378017 |
Target: 5'- uGGCGCUUGCCGAg--GCUGguaagaGGCUg -3' miRNA: 3'- gCUGCGAACGGUUacaCGGCag----CCGG- -5' |
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12374 | 5' | -55.8 | NC_003324.1 | + | 35350 | 0.73 | 0.304946 |
Target: 5'- -uGCGgUUGCUGuUGUGCCugGUCGGCCu -3' miRNA: 3'- gcUGCgAACGGUuACACGG--CAGCCGG- -5' |
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12374 | 5' | -55.8 | NC_003324.1 | + | 8330 | 0.78 | 0.163673 |
Target: 5'- uCGGCGCUcGUUAcgGUGaCCGUCGGCg -3' miRNA: 3'- -GCUGCGAaCGGUuaCAC-GGCAGCCGg -5' |
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12374 | 5' | -55.8 | NC_003324.1 | + | 34520 | 1.13 | 0.000539 |
Target: 5'- cCGACGCUUGCCAAUGUGCCGUCGGCCg -3' miRNA: 3'- -GCUGCGAACGGUUACACGGCAGCCGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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