Results 1 - 7 of 7 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
12375 | 3' | -51.7 | NC_003324.1 | + | 23098 | 0.66 | 0.862288 |
Target: 5'- cGGGCgccaAGGUCCUggcGACGAcugaGAAGGAu -3' miRNA: 3'- -CUCG----UCCGGGAa--CUGCUaaa-CUUCCU- -5' |
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12375 | 3' | -51.7 | NC_003324.1 | + | 28608 | 0.66 | 0.853713 |
Target: 5'- cGGCAGGCCUcagUGGucuCGGUccGAAGGGc -3' miRNA: 3'- cUCGUCCGGGa--ACU---GCUAaaCUUCCU- -5' |
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12375 | 3' | -51.7 | NC_003324.1 | + | 55577 | 0.66 | 0.853713 |
Target: 5'- cGGCAGGCuCCUgcccGGCGuggaagaaaUGGAGGAu -3' miRNA: 3'- cUCGUCCG-GGAa---CUGCuaa------ACUUCCU- -5' |
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12375 | 3' | -51.7 | NC_003324.1 | + | 57245 | 0.68 | 0.790345 |
Target: 5'- cGGCAGGCCCUUGGCuucgcacucagucGggGGc -3' miRNA: 3'- cUCGUCCGGGAACUGcuaaa--------CuuCCu -5' |
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12375 | 3' | -51.7 | NC_003324.1 | + | 16438 | 0.68 | 0.777026 |
Target: 5'- cGAGCAGGUCCUgGACGAcgcGAucGAc -3' miRNA: 3'- -CUCGUCCGGGAaCUGCUaaaCUucCU- -5' |
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12375 | 3' | -51.7 | NC_003324.1 | + | 48938 | 0.68 | 0.777026 |
Target: 5'- uGAGCGcaauGCCCUcugagcUGGCGA--UGAAGGAc -3' miRNA: 3'- -CUCGUc---CGGGA------ACUGCUaaACUUCCU- -5' |
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12375 | 3' | -51.7 | NC_003324.1 | + | 34854 | 1.1 | 0.001935 |
Target: 5'- cGAGCAGGCCCUUGACGAUUUGAAGGAu -3' miRNA: 3'- -CUCGUCCGGGAACUGCUAAACUUCCU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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