Results 41 - 49 of 49 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 12377 | 3' | -59.5 | NC_003324.1 | + | 39796 | 0.72 | 0.200839 |
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Target: 5'- uGGCUGCAGaacCGCAGGCCGGgCAc- -3' miRNA: 3'- -CCGACGUCgucGUGUCCGGCUgGUcc -5' |
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| 12377 | 3' | -59.5 | NC_003324.1 | + | 28980 | 0.73 | 0.180872 |
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Target: 5'- cGGUcGCAGCgaAGgACgAGGCCGGCCAGu -3' miRNA: 3'- -CCGaCGUCG--UCgUG-UCCGGCUGGUCc -5' |
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| 12377 | 3' | -59.5 | NC_003324.1 | + | 47543 | 0.74 | 0.143814 |
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Target: 5'- aGCUGCGGCAGUGCcaugguggaugacgaGGGCCGAUCGa- -3' miRNA: 3'- cCGACGUCGUCGUG---------------UCCGGCUGGUcc -5' |
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| 12377 | 3' | -59.5 | NC_003324.1 | + | 49419 | 0.75 | 0.120884 |
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Target: 5'- cGGCgagcccgGCAGCGGCGCuuGGCUGAUCuGGc -3' miRNA: 3'- -CCGa------CGUCGUCGUGu-CCGGCUGGuCC- -5' |
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| 12377 | 3' | -59.5 | NC_003324.1 | + | 1852 | 0.75 | 0.120884 |
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Target: 5'- uGCUGCAGCAGCAgcuucauuCuGG-CGGCCGGGa -3' miRNA: 3'- cCGACGUCGUCGU--------GuCCgGCUGGUCC- -5' |
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| 12377 | 3' | -59.5 | NC_003324.1 | + | 16137 | 0.76 | 0.105385 |
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Target: 5'- cGCUuCGGUAGCGgGGGCCGcGCCAGGc -3' miRNA: 3'- cCGAcGUCGUCGUgUCCGGC-UGGUCC- -5' |
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| 12377 | 3' | -59.5 | NC_003324.1 | + | 54063 | 0.77 | 0.091768 |
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Target: 5'- cGCUGCGGCGGaAUcGGCCGACCGGc -3' miRNA: 3'- cCGACGUCGUCgUGuCCGGCUGGUCc -5' |
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| 12377 | 3' | -59.5 | NC_003324.1 | + | 14347 | 0.78 | 0.079832 |
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Target: 5'- aGCUGCAGaAGCGCGGGCCGACg--- -3' miRNA: 3'- cCGACGUCgUCGUGUCCGGCUGgucc -5' |
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| 12377 | 3' | -59.5 | NC_003324.1 | + | 35449 | 1.11 | 0.000266 |
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Target: 5'- aGGCUGCAGCAGCACAGGCCGACCAGGc -3' miRNA: 3'- -CCGACGUCGUCGUGUCCGGCUGGUCC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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