miRNA display CGI


Results 1 - 3 of 3 are showing below:
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ID Location Perfect MFE.* RefSeq Acc. strand Start Position R_P_ratio# P value
Predicted miRNA align pattern
12378 3' -47.4 NC_003324.1 + 52484 0.66 0.987323
Target:  5'- uUCGCCGaucgGCUCGAcCUCGUa----- -3'
miRNA:   3'- uAGCGGUa---CGAGCUaGAGUAauaacu -5'
12378 3' -47.4 NC_003324.1 + 29263 0.68 0.938554
Target:  5'- cAUCGCCggGCUCGcgCUCGa------ -3'
miRNA:   3'- -UAGCGGuaCGAGCuaGAGUaauaacu -5'
12378 3' -47.4 NC_003324.1 + 35858 1.08 0.006534
Target:  5'- cAUCGCCAUGCUCGAUCUCAUUAUUGAg -3'
miRNA:   3'- -UAGCGGUACGAGCUAGAGUAAUAACU- -5'
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Note:
      When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.

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TDL, Institute of Biomedical Science, Academia Sinica, Taipei, Taiwan.
Copyright © 2007 TDL. All rights reserved.