miRNA: 3'- uAGCGGUa---CGAGCUaGAGUAauaacu -5'
miRNA: 3'- -UAGCGGuaCGAGCuaGAGUaauaacu -5'
miRNA: 3'- -UAGCGGUACGAGCUAGAGUAAUAACU- -5'
Results 1 - 3 of 3 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 12378 | 3' | -47.4 | NC_003324.1 | + | 52484 | 0.66 | 0.987323 |
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Target: 5'- uUCGCCGaucgGCUCGAcCUCGUa----- -3' miRNA: 3'- uAGCGGUa---CGAGCUaGAGUAauaacu -5' |
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| 12378 | 3' | -47.4 | NC_003324.1 | + | 29263 | 0.68 | 0.938554 |
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Target: 5'- cAUCGCCggGCUCGcgCUCGa------ -3' miRNA: 3'- -UAGCGGuaCGAGCuaGAGUaauaacu -5' |
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| 12378 | 3' | -47.4 | NC_003324.1 | + | 35858 | 1.08 | 0.006534 |
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Target: 5'- cAUCGCCAUGCUCGAUCUCAUUAUUGAg -3' miRNA: 3'- -UAGCGGUACGAGCUAGAGUAAUAACU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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