Results 1 - 8 of 8 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 12378 | 5' | -55.7 | NC_003324.1 | + | 6414 | 0.66 | 0.641797 |
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Target: 5'- -gUCUGCAGCGau-CcAGCUCGUGAAg -3' miRNA: 3'- uaAGGUGUCGCgcuGcUCGAGCACUU- -5' |
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| 12378 | 5' | -55.7 | NC_003324.1 | + | 7184 | 0.67 | 0.564052 |
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Target: 5'- --aUC-CAGCGCGcCGAGCUCGaUGGc -3' miRNA: 3'- uaaGGuGUCGCGCuGCUCGAGC-ACUu -5' |
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| 12378 | 5' | -55.7 | NC_003324.1 | + | 28067 | 0.67 | 0.564052 |
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Target: 5'- --aCCACGGCuuGCcgGAUGGGCUCGUGc- -3' miRNA: 3'- uaaGGUGUCG--CG--CUGCUCGAGCACuu -5' |
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| 12378 | 5' | -55.7 | NC_003324.1 | + | 18809 | 0.68 | 0.510017 |
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Target: 5'- -gUCCAUGGCGCGGCGaAGgUCGg--- -3' miRNA: 3'- uaAGGUGUCGCGCUGC-UCgAGCacuu -5' |
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| 12378 | 5' | -55.7 | NC_003324.1 | + | 23543 | 0.68 | 0.508957 |
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Target: 5'- --cCCACAGCGUGGCGGcgggcgcGCUCGg--- -3' miRNA: 3'- uaaGGUGUCGCGCUGCU-------CGAGCacuu -5' |
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| 12378 | 5' | -55.7 | NC_003324.1 | + | 23419 | 0.69 | 0.478647 |
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Target: 5'- --cCCGC--CGCGACGAGCUCGUcaGGAu -3' miRNA: 3'- uaaGGUGucGCGCUGCUCGAGCA--CUU- -5' |
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| 12378 | 5' | -55.7 | NC_003324.1 | + | 23680 | 0.7 | 0.400089 |
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Target: 5'- -aUCCACGGCGCGcCGGGCUUc---- -3' miRNA: 3'- uaAGGUGUCGCGCuGCUCGAGcacuu -5' |
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| 12378 | 5' | -55.7 | NC_003324.1 | + | 35822 | 1.04 | 0.001703 |
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Target: 5'- uAUUCCACAGCGCGACGAGCUCGUGAAg -3' miRNA: 3'- -UAAGGUGUCGCGCUGCUCGAGCACUU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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