Results 21 - 31 of 31 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
12379 | 3' | -56.7 | NC_003324.1 | + | 56211 | 0.66 | 0.67491 |
Target: 5'- cUCGCCUaucgcucgaucggCGGCGACaUCCUGcaUCcGUCCg -3' miRNA: 3'- -GGUGGA-------------GCCGCUGgAGGAC--AGcUAGG- -5' |
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12379 | 3' | -56.7 | NC_003324.1 | + | 25490 | 0.67 | 0.665364 |
Target: 5'- gUCGCCg-GGCGcuUCUCCUuGUCGAguuUCCg -3' miRNA: 3'- -GGUGGagCCGCu-GGAGGA-CAGCU---AGG- -5' |
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12379 | 3' | -56.7 | NC_003324.1 | + | 50703 | 0.67 | 0.644076 |
Target: 5'- cCCGCCUCGGCGGuaaCUCuaugCUGUgCGAa-- -3' miRNA: 3'- -GGUGGAGCCGCUg--GAG----GACA-GCUagg -5' |
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12379 | 3' | -56.7 | NC_003324.1 | + | 35665 | 0.67 | 0.644076 |
Target: 5'- uCCuuCUUGGCGGCUUCCUcgggGUuuucuucggccUGAUCCg -3' miRNA: 3'- -GGugGAGCCGCUGGAGGA----CA-----------GCUAGG- -5' |
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12379 | 3' | -56.7 | NC_003324.1 | + | 17537 | 0.67 | 0.633411 |
Target: 5'- cCCGCgUCGGCGAUCggaaCCaaa-GGUCCg -3' miRNA: 3'- -GGUGgAGCCGCUGGa---GGacagCUAGG- -5' |
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12379 | 3' | -56.7 | NC_003324.1 | + | 23794 | 0.67 | 0.633411 |
Target: 5'- uCgGCCUCGGCGAgcuuCUUCUUGaCGuugCCg -3' miRNA: 3'- -GgUGGAGCCGCU----GGAGGACaGCua-GG- -5' |
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12379 | 3' | -56.7 | NC_003324.1 | + | 19109 | 0.67 | 0.622746 |
Target: 5'- uCCuCUUCGGC-AUUgcgCUUGUCGAUCCc -3' miRNA: 3'- -GGuGGAGCCGcUGGa--GGACAGCUAGG- -5' |
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12379 | 3' | -56.7 | NC_003324.1 | + | 2995 | 0.68 | 0.601448 |
Target: 5'- gUCGCUUCGaGCGgcccaguaauucGCCUCUaugcgGUCGAUCUg -3' miRNA: 3'- -GGUGGAGC-CGC------------UGGAGGa----CAGCUAGG- -5' |
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12379 | 3' | -56.7 | NC_003324.1 | + | 32489 | 0.68 | 0.559208 |
Target: 5'- -aGCCUCGGCGAUCacggUGUCGAcgaugucgcUCCa -3' miRNA: 3'- ggUGGAGCCGCUGGagg-ACAGCU---------AGG- -5' |
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12379 | 3' | -56.7 | NC_003324.1 | + | 27971 | 0.68 | 0.548767 |
Target: 5'- gCACCUCGGUaccgGGCUUCacgaagaUGUCGAuauggUCCu -3' miRNA: 3'- gGUGGAGCCG----CUGGAGg------ACAGCU-----AGG- -5' |
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12379 | 3' | -56.7 | NC_003324.1 | + | 21861 | 0.69 | 0.507683 |
Target: 5'- gCCuCCUCGGCGGCCUuggCCUucuucgggGUCGAc-- -3' miRNA: 3'- -GGuGGAGCCGCUGGA---GGA--------CAGCUagg -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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