Results 1 - 20 of 23 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
12381 | 3' | -55 | NC_003324.1 | + | 38351 | 0.65 | 0.775095 |
Target: 5'- gGCACCGGGgcgGCGc---UGGUCGCCGu -3' miRNA: 3'- -CGUGGCCUa--UGCacaaGCCGGUGGUg -5' |
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12381 | 3' | -55 | NC_003324.1 | + | 41354 | 0.66 | 0.754832 |
Target: 5'- gGCGCUGGggGCGgcuuaaugaaGUagccggcaUCGGuCCGCCACu -3' miRNA: 3'- -CGUGGCCuaUGCa---------CA--------AGCC-GGUGGUG- -5' |
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12381 | 3' | -55 | NC_003324.1 | + | 30065 | 0.66 | 0.754832 |
Target: 5'- cCGCCGGucGCGacacCGGCCGCUGCg -3' miRNA: 3'- cGUGGCCuaUGCacaaGCCGGUGGUG- -5' |
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12381 | 3' | -55 | NC_003324.1 | + | 44306 | 0.66 | 0.753805 |
Target: 5'- -aACCGGGcGCgGUGgcgCGGCCgauagccucugcaGCCACg -3' miRNA: 3'- cgUGGCCUaUG-CACaa-GCCGG-------------UGGUG- -5' |
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12381 | 3' | -55 | NC_003324.1 | + | 23759 | 0.66 | 0.74139 |
Target: 5'- uGCGCCGGAaaucgggguccgauUcggcgGCGcGUUCGGCCuCgGCg -3' miRNA: 3'- -CGUGGCCU--------------A-----UGCaCAAGCCGGuGgUG- -5' |
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12381 | 3' | -55 | NC_003324.1 | + | 19053 | 0.66 | 0.734074 |
Target: 5'- cCGCuCGGu--CGU-UUCGGCCGCUACu -3' miRNA: 3'- cGUG-GCCuauGCAcAAGCCGGUGGUG- -5' |
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12381 | 3' | -55 | NC_003324.1 | + | 35362 | 0.67 | 0.717171 |
Target: 5'- uGUGCCuGGucgGCcUGUgcugcugcagccugcUCGGCCGCCGCg -3' miRNA: 3'- -CGUGG-CCua-UGcACA---------------AGCCGGUGGUG- -5' |
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12381 | 3' | -55 | NC_003324.1 | + | 42493 | 0.67 | 0.702204 |
Target: 5'- uGC-CCGGGUGCGcuucCGGUCcCCACg -3' miRNA: 3'- -CGuGGCCUAUGCacaaGCCGGuGGUG- -5' |
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12381 | 3' | -55 | NC_003324.1 | + | 40164 | 0.67 | 0.691431 |
Target: 5'- aGCAcCCGGAUuucgGCGccgcGUUcCGGCCAgacCCGCg -3' miRNA: 3'- -CGU-GGCCUA----UGCa---CAA-GCCGGU---GGUG- -5' |
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12381 | 3' | -55 | NC_003324.1 | + | 14704 | 0.67 | 0.680601 |
Target: 5'- -uGCCGGAaagggcCGUagcucCGGCCGCCACg -3' miRNA: 3'- cgUGGCCUau----GCAcaa--GCCGGUGGUG- -5' |
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12381 | 3' | -55 | NC_003324.1 | + | 36814 | 0.67 | 0.680601 |
Target: 5'- cGCAUCGGAUGa-UGUcacUGGCUACCAg -3' miRNA: 3'- -CGUGGCCUAUgcACAa--GCCGGUGGUg -5' |
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12381 | 3' | -55 | NC_003324.1 | + | 23996 | 0.67 | 0.680601 |
Target: 5'- cGCGCUGGuaggGCGgcgGUagGGCUGCUGCg -3' miRNA: 3'- -CGUGGCCua--UGCa--CAagCCGGUGGUG- -5' |
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12381 | 3' | -55 | NC_003324.1 | + | 1531 | 0.67 | 0.669725 |
Target: 5'- cGCGCCGGAUACG---UCGcGCCGuuuccuuugcgUCGCg -3' miRNA: 3'- -CGUGGCCUAUGCacaAGC-CGGU-----------GGUG- -5' |
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12381 | 3' | -55 | NC_003324.1 | + | 39716 | 0.68 | 0.658814 |
Target: 5'- uGC-CCGGccUGCG-GUUCugcaGCCACCACa -3' miRNA: 3'- -CGuGGCCu-AUGCaCAAGc---CGGUGGUG- -5' |
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12381 | 3' | -55 | NC_003324.1 | + | 55029 | 0.68 | 0.647879 |
Target: 5'- cGCGCCGGGcaggcaagGCGgacgGcUgGGCUGCCGCg -3' miRNA: 3'- -CGUGGCCUa-------UGCa---CaAgCCGGUGGUG- -5' |
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12381 | 3' | -55 | NC_003324.1 | + | 4477 | 0.69 | 0.574717 |
Target: 5'- gGCGCCGGAUGCGgcucacauccucuaCGGCgGCgACg -3' miRNA: 3'- -CGUGGCCUAUGCacaa----------GCCGgUGgUG- -5' |
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12381 | 3' | -55 | NC_003324.1 | + | 42197 | 0.69 | 0.571471 |
Target: 5'- uGCGCCGGA---GUGaUCGGCCcaacgcuuaacACCAUa -3' miRNA: 3'- -CGUGGCCUaugCACaAGCCGG-----------UGGUG- -5' |
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12381 | 3' | -55 | NC_003324.1 | + | 30133 | 0.69 | 0.571471 |
Target: 5'- cGCgACCGGcgGCGUGaUgGGCgGCgGCa -3' miRNA: 3'- -CG-UGGCCuaUGCACaAgCCGgUGgUG- -5' |
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12381 | 3' | -55 | NC_003324.1 | + | 21489 | 0.7 | 0.507744 |
Target: 5'- -uGCCGGAUgccgcgcccgucGCGUugcucaaggucGgaCGGCCGCCACg -3' miRNA: 3'- cgUGGCCUA------------UGCA-----------CaaGCCGGUGGUG- -5' |
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12381 | 3' | -55 | NC_003324.1 | + | 30067 | 0.71 | 0.477008 |
Target: 5'- uGCACCGGccgggaucgAUGCGgaccCGGCUGCCGCc -3' miRNA: 3'- -CGUGGCC---------UAUGCacaaGCCGGUGGUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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