Results 1 - 20 of 78 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
12381 | 5' | -63.6 | NC_003324.1 | + | 30151 | 0.66 | 0.370382 |
Target: 5'- aGC-CG-CaGCGGCCGGUGUCGCgaCCGg -3' miRNA: 3'- -CGuGCuGcCGCUGGUCGCGGCGg-GGC- -5' |
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12381 | 5' | -63.6 | NC_003324.1 | + | 49898 | 0.66 | 0.370382 |
Target: 5'- cGCuGCGuCGGCGcgcucgugcucGCCuuCGCUGCCCCc -3' miRNA: 3'- -CG-UGCuGCCGC-----------UGGucGCGGCGGGGc -5' |
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12381 | 5' | -63.6 | NC_003324.1 | + | 32119 | 0.66 | 0.370382 |
Target: 5'- gGC-CGAgugccuUGGUcuccgccucgGGCCGG-GCCGCCCCGg -3' miRNA: 3'- -CGuGCU------GCCG----------CUGGUCgCGGCGGGGC- -5' |
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12381 | 5' | -63.6 | NC_003324.1 | + | 55040 | 0.66 | 0.370382 |
Target: 5'- gGCAaGGCGGaCGGCUGG-GCUGCCgCGa -3' miRNA: 3'- -CGUgCUGCC-GCUGGUCgCGGCGGgGC- -5' |
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12381 | 5' | -63.6 | NC_003324.1 | + | 13015 | 0.66 | 0.370382 |
Target: 5'- uGCAgGGCGGC-AUCAaCGCCcGCCCUc -3' miRNA: 3'- -CGUgCUGCCGcUGGUcGCGG-CGGGGc -5' |
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12381 | 5' | -63.6 | NC_003324.1 | + | 5466 | 0.66 | 0.370382 |
Target: 5'- gGCA-GGCGGCG-UC-GC-CCGCCCCa -3' miRNA: 3'- -CGUgCUGCCGCuGGuCGcGGCGGGGc -5' |
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12381 | 5' | -63.6 | NC_003324.1 | + | 54339 | 0.66 | 0.370382 |
Target: 5'- gGCGCuGAugccgguccaUGGCaACCGGUGCCGCgCCu -3' miRNA: 3'- -CGUG-CU----------GCCGcUGGUCGCGGCGgGGc -5' |
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12381 | 5' | -63.6 | NC_003324.1 | + | 2545 | 0.66 | 0.362297 |
Target: 5'- -gGCGACGGaCGGCagaCGGCacGCCGUUCCa -3' miRNA: 3'- cgUGCUGCC-GCUG---GUCG--CGGCGGGGc -5' |
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12381 | 5' | -63.6 | NC_003324.1 | + | 326 | 0.66 | 0.362297 |
Target: 5'- -aACGAacCGGCGAUgCGGcCGCCaGCCCUa -3' miRNA: 3'- cgUGCU--GCCGCUG-GUC-GCGG-CGGGGc -5' |
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12381 | 5' | -63.6 | NC_003324.1 | + | 50691 | 0.66 | 0.354335 |
Target: 5'- --uCGACGGUGACCc---CCGCCUCGg -3' miRNA: 3'- cguGCUGCCGCUGGucgcGGCGGGGC- -5' |
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12381 | 5' | -63.6 | NC_003324.1 | + | 19273 | 0.66 | 0.354335 |
Target: 5'- -gGCGAUGGagauCGcCCAGCGCCGCgCa- -3' miRNA: 3'- cgUGCUGCC----GCuGGUCGCGGCGgGgc -5' |
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12381 | 5' | -63.6 | NC_003324.1 | + | 26925 | 0.66 | 0.354335 |
Target: 5'- gGCAUGGCGGCGAucgacgaggcacUCAGCggaGCgGCgCCa -3' miRNA: 3'- -CGUGCUGCCGCU------------GGUCG---CGgCGgGGc -5' |
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12381 | 5' | -63.6 | NC_003324.1 | + | 3927 | 0.66 | 0.354335 |
Target: 5'- cGCAUGACGuGCGGCuCAaCGaaggaGCCCCa -3' miRNA: 3'- -CGUGCUGC-CGCUG-GUcGCgg---CGGGGc -5' |
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12381 | 5' | -63.6 | NC_003324.1 | + | 18906 | 0.66 | 0.346497 |
Target: 5'- gGCGUGAUGGaGACUGGCGCCGaCCUu -3' miRNA: 3'- -CGUGCUGCCgCUGGUCGCGGCgGGGc -5' |
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12381 | 5' | -63.6 | NC_003324.1 | + | 53483 | 0.66 | 0.346497 |
Target: 5'- cGCGCGGaagccgaGGCcgaauGCCGGCGauGCCCUGa -3' miRNA: 3'- -CGUGCUg------CCGc----UGGUCGCggCGGGGC- -5' |
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12381 | 5' | -63.6 | NC_003324.1 | + | 53542 | 0.66 | 0.346497 |
Target: 5'- ---gGACGGCGAUCA-CGuCCGCUUCGa -3' miRNA: 3'- cgugCUGCCGCUGGUcGC-GGCGGGGC- -5' |
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12381 | 5' | -63.6 | NC_003324.1 | + | 34060 | 0.66 | 0.338784 |
Target: 5'- aUACccACGGCGcccaauGCCAGCGgcuCCGCCCUGu -3' miRNA: 3'- cGUGc-UGCCGC------UGGUCGC---GGCGGGGC- -5' |
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12381 | 5' | -63.6 | NC_003324.1 | + | 18043 | 0.66 | 0.338784 |
Target: 5'- -uGCGAUaGGCGGCgAGUGCCGUCaCUu -3' miRNA: 3'- cgUGCUG-CCGCUGgUCGCGGCGG-GGc -5' |
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12381 | 5' | -63.6 | NC_003324.1 | + | 5096 | 0.66 | 0.338784 |
Target: 5'- gGCGCGAuCGaCGAUCuGCGCCGCUUUu -3' miRNA: 3'- -CGUGCU-GCcGCUGGuCGCGGCGGGGc -5' |
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12381 | 5' | -63.6 | NC_003324.1 | + | 49049 | 0.66 | 0.338784 |
Target: 5'- cCGCGGCGGCGGggcaauccauCCGGCuCUGCaCCGg -3' miRNA: 3'- cGUGCUGCCGCU----------GGUCGcGGCGgGGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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