Results 1 - 20 of 25 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
12382 | 3' | -56.4 | NC_003324.1 | + | 42383 | 0.66 | 0.665939 |
Target: 5'- cGUCGuAUCGCgguaucCUGgUCGCGGGuCCGCAa -3' miRNA: 3'- -CAGU-UAGCG------GGCgAGCGUCUuGGCGU- -5' |
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12382 | 3' | -56.4 | NC_003324.1 | + | 4575 | 0.66 | 0.665939 |
Target: 5'- aGUCGcGUCG-CCGC-CGUAGAggaugugaGCCGCAu -3' miRNA: 3'- -CAGU-UAGCgGGCGaGCGUCU--------UGGCGU- -5' |
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12382 | 3' | -56.4 | NC_003324.1 | + | 49445 | 0.66 | 0.655003 |
Target: 5'- uUCGagGUCGCCCGaUCGUcGAcACCGCu -3' miRNA: 3'- cAGU--UAGCGGGCgAGCGuCU-UGGCGu -5' |
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12382 | 3' | -56.4 | NC_003324.1 | + | 3030 | 0.66 | 0.62211 |
Target: 5'- gGUCGAUCugcucuuggGCCUGUUCGCGGG--CGCGg -3' miRNA: 3'- -CAGUUAG---------CGGGCGAGCGUCUugGCGU- -5' |
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12382 | 3' | -56.4 | NC_003324.1 | + | 14719 | 0.66 | 0.62211 |
Target: 5'- uGUUggUCGCCUGaUUGCcggaaAGGGCCGUAg -3' miRNA: 3'- -CAGuuAGCGGGCgAGCG-----UCUUGGCGU- -5' |
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12382 | 3' | -56.4 | NC_003324.1 | + | 10980 | 0.67 | 0.600209 |
Target: 5'- cGUCGuuuauGUCgGCCUGCUCGUAGAcaACCa-- -3' miRNA: 3'- -CAGU-----UAG-CGGGCGAGCGUCU--UGGcgu -5' |
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12382 | 3' | -56.4 | NC_003324.1 | + | 52480 | 0.67 | 0.600209 |
Target: 5'- -cCGAUCgGCUCGacCUCGUAGAcuuuGCCGCGg -3' miRNA: 3'- caGUUAG-CGGGC--GAGCGUCU----UGGCGU- -5' |
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12382 | 3' | -56.4 | NC_003324.1 | + | 48620 | 0.67 | 0.567581 |
Target: 5'- gGUCGG-CGCCCGCaUC-CGGcuccGCCGCAa -3' miRNA: 3'- -CAGUUaGCGGGCG-AGcGUCu---UGGCGU- -5' |
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12382 | 3' | -56.4 | NC_003324.1 | + | 8569 | 0.68 | 0.546075 |
Target: 5'- aUCGAuuUCGCgCGCUgGCucacugccgAGGACCGCGu -3' miRNA: 3'- cAGUU--AGCGgGCGAgCG---------UCUUGGCGU- -5' |
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12382 | 3' | -56.4 | NC_003324.1 | + | 37339 | 0.68 | 0.546075 |
Target: 5'- aUCAAUCGCCUGCUCuCGGcGAUUGUc -3' miRNA: 3'- cAGUUAGCGGGCGAGcGUC-UUGGCGu -5' |
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12382 | 3' | -56.4 | NC_003324.1 | + | 31154 | 0.68 | 0.535419 |
Target: 5'- ------gGCCUGcCUCGCGGAGCUGCc -3' miRNA: 3'- caguuagCGGGC-GAGCGUCUUGGCGu -5' |
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12382 | 3' | -56.4 | NC_003324.1 | + | 54976 | 0.68 | 0.493597 |
Target: 5'- cUCAAUCuCUCGCUUGCGGGcGCCGaCAu -3' miRNA: 3'- cAGUUAGcGGGCGAGCGUCU-UGGC-GU- -5' |
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12382 | 3' | -56.4 | NC_003324.1 | + | 7280 | 0.68 | 0.493597 |
Target: 5'- -aCGGUCGCUCGCUacaucaCGCAGAuccagcccaacACCGUg -3' miRNA: 3'- caGUUAGCGGGCGA------GCGUCU-----------UGGCGu -5' |
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12382 | 3' | -56.4 | NC_003324.1 | + | 16353 | 0.69 | 0.473251 |
Target: 5'- cGUCGAUCGCgucguccaggacCUGCUCGCA-AACgGCAu -3' miRNA: 3'- -CAGUUAGCG------------GGCGAGCGUcUUGgCGU- -5' |
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12382 | 3' | -56.4 | NC_003324.1 | + | 20475 | 0.69 | 0.473251 |
Target: 5'- cUCGAUCG-CCGCaUCGCGGAGCgGgAu -3' miRNA: 3'- cAGUUAGCgGGCG-AGCGUCUUGgCgU- -5' |
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12382 | 3' | -56.4 | NC_003324.1 | + | 2773 | 0.7 | 0.424341 |
Target: 5'- uUUGAUCGCCCGauCUgGCAGGucuACCGCc -3' miRNA: 3'- cAGUUAGCGGGC--GAgCGUCU---UGGCGu -5' |
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12382 | 3' | -56.4 | NC_003324.1 | + | 24498 | 0.7 | 0.414926 |
Target: 5'- uUCGcAUCGCCCGacgCGCAGugcauauCCGCAu -3' miRNA: 3'- cAGU-UAGCGGGCga-GCGUCuu-----GGCGU- -5' |
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12382 | 3' | -56.4 | NC_003324.1 | + | 13696 | 0.7 | 0.387464 |
Target: 5'- -cCGAUCGCCUGCgcgucuUCGCGGAAuUUGCAg -3' miRNA: 3'- caGUUAGCGGGCG------AGCGUCUU-GGCGU- -5' |
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12382 | 3' | -56.4 | NC_003324.1 | + | 5165 | 0.71 | 0.344434 |
Target: 5'- cGUCGAUCGCgCCGCUUaaGCuaucaGGCCGCGu -3' miRNA: 3'- -CAGUUAGCG-GGCGAG--CGuc---UUGGCGU- -5' |
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12382 | 3' | -56.4 | NC_003324.1 | + | 13489 | 0.71 | 0.336251 |
Target: 5'- cUCAGUCGCCUGUaucUgGCcGAGCUGCAg -3' miRNA: 3'- cAGUUAGCGGGCG---AgCGuCUUGGCGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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