Results 1 - 7 of 7 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 12383 | 5' | -62.3 | NC_003324.1 | + | 13866 | 0.66 | 0.37886 |
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Target: 5'- -cGUGCgggagGGCGUaauGCGCCAGGCaGGu -3' miRNA: 3'- acCACGa----CUGCAc--CGCGGUCCGcCCu -5' |
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| 12383 | 5' | -62.3 | NC_003324.1 | + | 23537 | 0.67 | 0.307842 |
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Target: 5'- cGGUGCcccacaGCGUGGCGgCGGGCGc-- -3' miRNA: 3'- aCCACGac----UGCACCGCgGUCCGCccu -5' |
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| 12383 | 5' | -62.3 | NC_003324.1 | + | 17357 | 0.69 | 0.247454 |
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Target: 5'- aGcGUGCaUGACGUGGuCGCaCAGGCGc-- -3' miRNA: 3'- aC-CACG-ACUGCACC-GCG-GUCCGCccu -5' |
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| 12383 | 5' | -62.3 | NC_003324.1 | + | 31315 | 0.69 | 0.235442 |
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Target: 5'- cGGccgGC-GGCGcUGGCGCU-GGCGGGGg -3' miRNA: 3'- aCCa--CGaCUGC-ACCGCGGuCCGCCCU- -5' |
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| 12383 | 5' | -62.3 | NC_003324.1 | + | 20609 | 0.7 | 0.197211 |
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Target: 5'- cGGcGCUGAUG-GGCGCUugcggucGGCGGGc -3' miRNA: 3'- aCCaCGACUGCaCCGCGGu------CCGCCCu -5' |
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| 12383 | 5' | -62.3 | NC_003324.1 | + | 9668 | 0.75 | 0.084057 |
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Target: 5'- cUGG-GC-GGCGUGGgGCCGGGCGGa- -3' miRNA: 3'- -ACCaCGaCUGCACCgCGGUCCGCCcu -5' |
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| 12383 | 5' | -62.3 | NC_003324.1 | + | 39648 | 1.08 | 0.000269 |
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Target: 5'- cUGGUGCUGACGUGGCGCCAGGCGGGAc -3' miRNA: 3'- -ACCACGACUGCACCGCGGUCCGCCCU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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