Results 1 - 10 of 10 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 12384 | 5' | -56.8 | NC_003324.1 | + | 15385 | 0.66 | 0.614937 |
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Target: 5'- cGAGCgCAGCGagcaGAAGCgCGGUGAGc-- -3' miRNA: 3'- -CUCG-GUCGUgg--CUUCG-GCCACUUcaa -5' |
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| 12384 | 5' | -56.8 | NC_003324.1 | + | 38981 | 0.66 | 0.614937 |
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Target: 5'- -cGCUGGCgcuuGCCGAGGCUGGUaaGAGGc- -3' miRNA: 3'- cuCGGUCG----UGGCUUCGGCCA--CUUCaa -5' |
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| 12384 | 5' | -56.8 | NC_003324.1 | + | 38997 | 0.66 | 0.58182 |
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Target: 5'- cAGCC-GCAgCGAgugAGCCGGUGAc--- -3' miRNA: 3'- cUCGGuCGUgGCU---UCGGCCACUucaa -5' |
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| 12384 | 5' | -56.8 | NC_003324.1 | + | 38098 | 0.67 | 0.516938 |
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Target: 5'- -cGUCGGCGCCGu-GCUGGUGAc--- -3' miRNA: 3'- cuCGGUCGUGGCuuCGGCCACUucaa -5' |
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| 12384 | 5' | -56.8 | NC_003324.1 | + | 42311 | 0.68 | 0.465178 |
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Target: 5'- -uGCCAcGUGCCGGGcGCCGGUGAcAGa- -3' miRNA: 3'- cuCGGU-CGUGGCUU-CGGCCACU-UCaa -5' |
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| 12384 | 5' | -56.8 | NC_003324.1 | + | 14479 | 0.68 | 0.445235 |
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Target: 5'- -uGCUGGCACCgucgcgGAAGCCGGUGcAGc- -3' miRNA: 3'- cuCGGUCGUGG------CUUCGGCCACuUCaa -5' |
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| 12384 | 5' | -56.8 | NC_003324.1 | + | 35123 | 0.69 | 0.425779 |
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Target: 5'- -cGCCAGCGCCGucGCCGuUGAc--- -3' miRNA: 3'- cuCGGUCGUGGCuuCGGCcACUucaa -5' |
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| 12384 | 5' | -56.8 | NC_003324.1 | + | 26124 | 0.69 | 0.416243 |
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Target: 5'- uGAGCUagAGCGCCGGcAGaCCGGUGAc--- -3' miRNA: 3'- -CUCGG--UCGUGGCU-UC-GGCCACUucaa -5' |
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| 12384 | 5' | -56.8 | NC_003324.1 | + | 40482 | 0.71 | 0.304953 |
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Target: 5'- -cGCCAGacgaaGgCGAAgGCCGGUGAGGUUu -3' miRNA: 3'- cuCGGUCg----UgGCUU-CGGCCACUUCAA- -5' |
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| 12384 | 5' | -56.8 | NC_003324.1 | + | 40304 | 1.04 | 0.001258 |
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Target: 5'- gGAGCCAGCACCGAAGCCGGUGAAGUUc -3' miRNA: 3'- -CUCGGUCGUGGCUUCGGCCACUUCAA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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