miRNA display CGI


Results 1 - 8 of 8 are showing below:
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ID Location Perfect MFE.* RefSeq Acc. strand Start Position R_P_ratio# P value
Predicted miRNA align pattern
12385 3' -55.1 NC_003324.1 + 40653 1.06 0.001802
Target:  5'- cAUCGGGCGACAUGACGACACGGUAGUg -3'
miRNA:   3'- -UAGCCCGCUGUACUGCUGUGCCAUCA- -5'
12385 3' -55.1 NC_003324.1 + 24993 0.76 0.229037
Target:  5'- -cCGGGCGGCGUGcUGGCgAUGGUGGUg -3'
miRNA:   3'- uaGCCCGCUGUACuGCUG-UGCCAUCA- -5'
12385 3' -55.1 NC_003324.1 + 17488 0.71 0.423009
Target:  5'- --aGGGCGACGUGGC-ACGCGGa--- -3'
miRNA:   3'- uagCCCGCUGUACUGcUGUGCCauca -5'
12385 3' -55.1 NC_003324.1 + 6238 0.69 0.56671
Target:  5'- cGUCGGGCagcGCAUuccaGGCGGCGCGGgucAGUc -3'
miRNA:   3'- -UAGCCCGc--UGUA----CUGCUGUGCCa--UCA- -5'
12385 3' -55.1 NC_003324.1 + 30136 0.67 0.653397
Target:  5'- uGUCGcgaccGGCGGCGUGaugggcgGCGGCAUgGGUGGUg -3'
miRNA:   3'- -UAGC-----CCGCUGUAC-------UGCUGUG-CCAUCA- -5'
12385 3' -55.1 NC_003324.1 + 13718 0.67 0.676433
Target:  5'- cGUCGgacGGCGGCAggcacugGAUGACGCGGg--- -3'
miRNA:   3'- -UAGC---CCGCUGUa------CUGCUGUGCCauca -5'
12385 3' -55.1 NC_003324.1 + 14901 0.66 0.698201
Target:  5'- --aGGGCGACGcgcuCGACGCGGUc-- -3'
miRNA:   3'- uagCCCGCUGUacu-GCUGUGCCAuca -5'
12385 3' -55.1 NC_003324.1 + 7341 0.66 0.730343
Target:  5'- --aGGGC-ACGcUGACGAUGCGGUuGGUa -3'
miRNA:   3'- uagCCCGcUGU-ACUGCUGUGCCA-UCA- -5'
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Note:
      When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.

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TDL, Institute of Biomedical Science, Academia Sinica, Taipei, Taiwan.
Copyright © 2007 TDL. All rights reserved.