Results 1 - 12 of 12 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
12386 | 3' | -55.7 | NC_003324.1 | + | 12213 | 0.66 | 0.711344 |
Target: 5'- gAUGUUgUCgGUgaaauucccgGCCGAggCCGCGCGc -3' miRNA: 3'- gUGCAAgAGgCAa---------CGGCUa-GGCGCGU- -5' |
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12386 | 3' | -55.7 | NC_003324.1 | + | 12328 | 0.66 | 0.700665 |
Target: 5'- aACGUcggCUCCGacaUUGUCGGcauugCCGCGCu -3' miRNA: 3'- gUGCAa--GAGGC---AACGGCUa----GGCGCGu -5' |
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12386 | 3' | -55.7 | NC_003324.1 | + | 10669 | 0.67 | 0.646506 |
Target: 5'- gACGguugCCGUUGCCuGcAUCCGCuGCAc -3' miRNA: 3'- gUGCaagaGGCAACGG-C-UAGGCG-CGU- -5' |
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12386 | 3' | -55.7 | NC_003324.1 | + | 2715 | 0.67 | 0.624679 |
Target: 5'- gGCGgcagUCUCCaagcgauaGCCGAUCgCGCGCc -3' miRNA: 3'- gUGCa---AGAGGcaa-----CGGCUAG-GCGCGu -5' |
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12386 | 3' | -55.7 | NC_003324.1 | + | 33362 | 0.67 | 0.624679 |
Target: 5'- uGCGUg----GUUGCCGGcUCCGCGCGc -3' miRNA: 3'- gUGCAagaggCAACGGCU-AGGCGCGU- -5' |
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12386 | 3' | -55.7 | NC_003324.1 | + | 27634 | 0.67 | 0.624679 |
Target: 5'- uGCGUgagCCGgcaGCCGAgcccgaaacaggUCCGCGCGc -3' miRNA: 3'- gUGCAagaGGCaa-CGGCU------------AGGCGCGU- -5' |
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12386 | 3' | -55.7 | NC_003324.1 | + | 37419 | 0.68 | 0.59201 |
Target: 5'- gCACGUUCU-UGUUGCuauCGAgcgcauugCCGCGCAc -3' miRNA: 3'- -GUGCAAGAgGCAACG---GCUa-------GGCGCGU- -5' |
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12386 | 3' | -55.7 | NC_003324.1 | + | 42223 | 0.68 | 0.59201 |
Target: 5'- cCAUGg--UCUGUUGCUGAUCCcCGCGg -3' miRNA: 3'- -GUGCaagAGGCAACGGCUAGGcGCGU- -5' |
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12386 | 3' | -55.7 | NC_003324.1 | + | 42377 | 0.72 | 0.345049 |
Target: 5'- gCACGUggcaggcggcgcacUCagCGUUGCCGAcCCGCGCc -3' miRNA: 3'- -GUGCA--------------AGagGCAACGGCUaGGCGCGu -5' |
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12386 | 3' | -55.7 | NC_003324.1 | + | 30760 | 0.73 | 0.323359 |
Target: 5'- gCugGUUUggcCCGgcgugGCgCGAUCCGCGCAg -3' miRNA: 3'- -GugCAAGa--GGCaa---CG-GCUAGGCGCGU- -5' |
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12386 | 3' | -55.7 | NC_003324.1 | + | 43079 | 0.85 | 0.047769 |
Target: 5'- --gGUUCUCCGUUGCCGAcCCGCGUg -3' miRNA: 3'- gugCAAGAGGCAACGGCUaGGCGCGu -5' |
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12386 | 3' | -55.7 | NC_003324.1 | + | 42845 | 1.08 | 0.001199 |
Target: 5'- gCACGUUCUCCGUUGCCGAUCCGCGCAu -3' miRNA: 3'- -GUGCAAGAGGCAACGGCUAGGCGCGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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