Results 41 - 46 of 46 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 12388 | 3' | -56.2 | NC_003324.1 | + | 21086 | 0.66 | 0.643491 |
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Target: 5'- uUUC-GGCG-CGGCCAUCauugcGGCGAuCCGAc -3' miRNA: 3'- -AAGuUUGCgGCCGGUAG-----UCGCU-GGCU- -5' |
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| 12388 | 3' | -56.2 | NC_003324.1 | + | 11143 | 0.66 | 0.654496 |
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Target: 5'- gUCcgcuuCGCCGGUCu--GGCGGCCGGc -3' miRNA: 3'- aAGuuu--GCGGCCGGuagUCGCUGGCU- -5' |
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| 12388 | 3' | -56.2 | NC_003324.1 | + | 29991 | 0.66 | 0.654496 |
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Target: 5'- ----cGCGCCguccagcauGGCCAgCAGCGcGCCGAa -3' miRNA: 3'- aaguuUGCGG---------CCGGUaGUCGC-UGGCU- -5' |
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| 12388 | 3' | -56.2 | NC_003324.1 | + | 32474 | 0.66 | 0.66548 |
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Target: 5'- gUCGccuuCGCCGGCagccUCGGCGAUCa- -3' miRNA: 3'- aAGUuu--GCGGCCGgu--AGUCGCUGGcu -5' |
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| 12388 | 3' | -56.2 | NC_003324.1 | + | 21388 | 0.66 | 0.66548 |
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Target: 5'- aUCGAGgauauCGCCGGCgAggcggCGaacGCGGCCGAg -3' miRNA: 3'- aAGUUU-----GCGGCCGgUa----GU---CGCUGGCU- -5' |
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| 12388 | 3' | -56.2 | NC_003324.1 | + | 55534 | 0.66 | 0.676433 |
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Target: 5'- gUCGAGCGCCaa--AUCAGCGugCGu -3' miRNA: 3'- aAGUUUGCGGccggUAGUCGCugGCu -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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