Results 1 - 20 of 34 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
12389 | 5' | -54.9 | NC_003324.1 | + | 20309 | 0.66 | 0.726935 |
Target: 5'- cCCG-GGCuGCAuUCCaUC-UGGGCUGCCg -3' miRNA: 3'- -GGCgUCGuCGU-AGG-AGcACUCGAUGG- -5' |
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12389 | 5' | -54.9 | NC_003324.1 | + | 38648 | 0.66 | 0.726935 |
Target: 5'- aCCGCAucacGC-GCGUgCCcuaUCGcccgGAGCUGCCg -3' miRNA: 3'- -GGCGU----CGuCGUA-GG---AGCa---CUCGAUGG- -5' |
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12389 | 5' | -54.9 | NC_003324.1 | + | 53293 | 0.66 | 0.737471 |
Target: 5'- uCCGCAGCuu--UCgUUGUGAagccggccgccaGCUACCa -3' miRNA: 3'- -GGCGUCGucguAGgAGCACU------------CGAUGG- -5' |
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12389 | 5' | -54.9 | NC_003324.1 | + | 6976 | 0.66 | 0.737471 |
Target: 5'- aCCGCGGUGGCGaagagCUgauagUGUGAuGUUGCCu -3' miRNA: 3'- -GGCGUCGUCGUa----GGa----GCACU-CGAUGG- -5' |
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12389 | 5' | -54.9 | NC_003324.1 | + | 25294 | 0.66 | 0.746862 |
Target: 5'- aCCGuCAGuCGGCGcaggcaacuUCCUUggucucaugccugGUGAGCUGCg -3' miRNA: 3'- -GGC-GUC-GUCGU---------AGGAG-------------CACUCGAUGg -5' |
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12389 | 5' | -54.9 | NC_003324.1 | + | 17200 | 0.66 | 0.758213 |
Target: 5'- aCGCGGCuuucGGCGUUCU--UGAGC-GCCg -3' miRNA: 3'- gGCGUCG----UCGUAGGAgcACUCGaUGG- -5' |
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12389 | 5' | -54.9 | NC_003324.1 | + | 34990 | 0.66 | 0.7479 |
Target: 5'- uCCGagccuGCAGCGUUUgCGUuuGCUGCCg -3' miRNA: 3'- -GGCgu---CGUCGUAGGaGCAcuCGAUGG- -5' |
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12389 | 5' | -54.9 | NC_003324.1 | + | 41916 | 0.66 | 0.737471 |
Target: 5'- gUCGCAGUcgccGCAUCCg---GAGgUACCg -3' miRNA: 3'- -GGCGUCGu---CGUAGGagcaCUCgAUGG- -5' |
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12389 | 5' | -54.9 | NC_003324.1 | + | 1851 | 0.66 | 0.726935 |
Target: 5'- gCUGCAGCAGCAgCUUCauucuGGCgGCCg -3' miRNA: 3'- -GGCGUCGUCGUaGGAGcac--UCGaUGG- -5' |
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12389 | 5' | -54.9 | NC_003324.1 | + | 44827 | 0.67 | 0.683965 |
Target: 5'- -gGgGGCGGCGUCauuggCGUGcccgcuGCUGCCg -3' miRNA: 3'- ggCgUCGUCGUAGga---GCACu-----CGAUGG- -5' |
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12389 | 5' | -54.9 | NC_003324.1 | + | 15752 | 0.67 | 0.705593 |
Target: 5'- cCUGaCGGUGGU---CUCGUGGGUUACCg -3' miRNA: 3'- -GGC-GUCGUCGuagGAGCACUCGAUGG- -5' |
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12389 | 5' | -54.9 | NC_003324.1 | + | 21090 | 0.67 | 0.683965 |
Target: 5'- gCCGCGGC-GCGUgCUgCGUcGGCUuCCa -3' miRNA: 3'- -GGCGUCGuCGUAgGA-GCAcUCGAuGG- -5' |
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12389 | 5' | -54.9 | NC_003324.1 | + | 43131 | 0.67 | 0.671979 |
Target: 5'- cCCGCAGguuGcCGUCCUCGacggcgagcuuguUGAGCgagcGCCa -3' miRNA: 3'- -GGCGUCgu-C-GUAGGAGC-------------ACUCGa---UGG- -5' |
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12389 | 5' | -54.9 | NC_003324.1 | + | 21851 | 0.67 | 0.662139 |
Target: 5'- gCGCAGCcgGGCcUCCUCGgcGGCcuugGCCu -3' miRNA: 3'- gGCGUCG--UCGuAGGAGCacUCGa---UGG- -5' |
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12389 | 5' | -54.9 | NC_003324.1 | + | 21081 | 0.67 | 0.673071 |
Target: 5'- gCGCGGCcaucauugcGGCgAUCCgaCGUGAGCuUAUCg -3' miRNA: 3'- gGCGUCG---------UCG-UAGGa-GCACUCG-AUGG- -5' |
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12389 | 5' | -54.9 | NC_003324.1 | + | 4805 | 0.67 | 0.683965 |
Target: 5'- gCCGUAGCAGCcgCCUUcUGgauuuccagccAGCcGCCc -3' miRNA: 3'- -GGCGUCGUCGuaGGAGcAC-----------UCGaUGG- -5' |
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12389 | 5' | -54.9 | NC_003324.1 | + | 45981 | 0.67 | 0.705593 |
Target: 5'- -aGCGGCGGCG-CCgaaUCGagGAGCgUGCCc -3' miRNA: 3'- ggCGUCGUCGUaGG---AGCa-CUCG-AUGG- -5' |
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12389 | 5' | -54.9 | NC_003324.1 | + | 54316 | 0.68 | 0.629217 |
Target: 5'- aCCGguGCcGCGcCUUCGcuuUGAGCaGCCg -3' miRNA: 3'- -GGCguCGuCGUaGGAGC---ACUCGaUGG- -5' |
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12389 | 5' | -54.9 | NC_003324.1 | + | 8163 | 0.68 | 0.629217 |
Target: 5'- aCCGUGGCAGCcauggucaccUCCUCGacgaAGUUGCCc -3' miRNA: 3'- -GGCGUCGUCGu---------AGGAGCac--UCGAUGG- -5' |
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12389 | 5' | -54.9 | NC_003324.1 | + | 13514 | 0.68 | 0.642398 |
Target: 5'- uCCGCGGCGGCGgugcacuggacaCCUCaGUcgccuguaucuggccGAGCUGCa -3' miRNA: 3'- -GGCGUCGUCGUa-----------GGAG-CA---------------CUCGAUGg -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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